Lu S X, Zhang M S, Luo F Q, Feng L, Tian Y A
Zhonghua Zhong Liu Za Zhi. 1986 Sep;8(5):328-31.
Radioimmunoassay of monoclonal antibodies against O6-methyldeoxyguanosine (O6-MedG) was used to detect the presence of these DNA adducts in the human esophageal epithelium. The analysis comprised 48 adjacent epithelial specimens of the esophageal and cardiac cancer resected in Linxian County and 30 specimens of the fetal esophageal epithelium and 4 of the normal esophageal epithelium from autopsy as collected from the hospital in Beijing. The results show that O6-MedG was detected in all the specimens from the esophageal and cardiac cancer patients. In 7 samples in the adjacent epithelium of esophageal cancer, the level of O6-MedG ranged from 0.5 to 1.0 pmol/mgDNA. 19 showed higher levels up to 37.4 pmol/mgDNA with a mean of 4.72 +/- 6.08 pmol/mgDNA. 5 samples of gastric mucosa showed the level of O6-MedG ranging 0.3-1.0 pmol/mgDNA and the remaining 6 showed a higher level of 1.2-13.4 pmol/mgDNA. The mean was 3.31 +/- 3.97. In all the 11 patients, O6-MedG was detected in the para-cancerous gastric mucosa of the cardiac cancer. 4 normal autopsied esophageal epithelial samples were too low for detection. Samples from the fetal esophageal epithelium showed lower level of O6-MedG, the mean was 0.4 +/- 0.57 pmol/mgDNA. The results mentioned above give us the new evidence that the effect of N-nitrosamines is most likely a causative factor in the carcinogenesis of human esophageal cancer.
采用抗O6-甲基脱氧鸟苷（O6-MedG）单克隆抗体的放射免疫分析法检测人食管上皮中这些DNA加合物的存在情况。分析的样本包括48例来自林县切除的食管癌和贲门癌的相邻上皮标本、30例胎儿食管上皮标本以及4例取自北京某医院尸检的正常食管上皮标本。结果显示，在所有食管癌和贲门癌患者的标本中均检测到O6-MedG。在7例食管癌相邻上皮样本中，O6-MedG水平为0.5至1.0 pmol/mgDNA。19例显示更高水平，最高达37.4 pmol/mgDNA，平均为4.72±6.08 pmol/mgDNA。5例胃黏膜样本中O6-MedG水平为0.3 - 1.0 pmol/mgDNA，其余6例显示更高水平，为1.2 - 13.4 pmol/mgDNA，平均为3.31±3.97。在所有11例患者的贲门癌癌旁胃黏膜中均检测到O6-MedG。4例正常尸检食管上皮样本因含量过低而无法检测。胎儿食管上皮样本显示O6-MedG水平较低，平均为0.4±0.57 pmol/mgDNA。上述结果为我们提供了新的证据，表明亚硝胺的作用很可能是人类食管癌致癌过程中的一个致病因素。
