The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin 150030, China.
The Laboratory of Cell and Developmental Biology, Northeast Agricultural University, Harbin 150030, China.
Anim Reprod Sci. 2022 Aug;243:107015. doi: 10.1016/j.anireprosci.2022.107015. Epub 2022 Jun 6.
Pregnancy success is closely related to the molecular mechanisms that control energy metabolism balance. However, the mechanisms have not been fully understood. Uncoupling protein 2 (UCP2) plays a physiological role by regulating energy metabolism in numerous tissues. In this study, we determined the expression and hormone regulation of UCP2 in goat uterus. UCP2 is expressed in the luminal and glandular epithelia of goat uterus during early pregnancy, as revealed by in situ hybridization and immunohistochemistry conducted on pregnant goats. The signals were detected from day 0 (D0) to D30 of pregnancy, though weak on D16 (the adhesion period). The low levels of UCP2 on D16 were confirmed by RT-qPCR and western blot. In goat uterus and endometrial epithelial cells (EECs), UCP2 was up-regulated by progesterone and estrogen. In addition, after goat EECs were treated with genipin (an inhibitor of UCP2), not only UCP2 expression but also cell proliferation was inhibited. Collectively, UCP2 is dynamically expressed in goat uterus and can affect EEC proliferation, suggesting that it may participate in regulating the energy metabolism balance of goat uterus during early pregnancy.
妊娠成功与控制能量代谢平衡的分子机制密切相关。然而，这些机制尚未得到充分理解。解偶联蛋白 2（UCP2）通过调节许多组织中的能量代谢发挥生理作用。在这项研究中，我们确定了 UCP2 在山羊子宫中的表达和激素调节。通过对妊娠山羊进行原位杂交和免疫组织化学检测，发现 UCP2 在妊娠早期山羊子宫的腔上皮和腺上皮中表达。信号从妊娠第 0 天（D0）到第 30 天（D30）检测到，但在第 16 天（黏附期）较弱。D16 时 UCP2 水平较低，通过 RT-qPCR 和 western blot 得到证实。在山羊子宫和子宫内膜上皮细胞（EEC）中，孕激素和雌激素上调 UCP2。此外，在山羊 EEC 用京尼平（UCP2 的抑制剂）处理后，不仅 UCP2 的表达，而且细胞增殖也受到抑制。总之，UCP2 在山羊子宫中动态表达，并能影响 EEC 的增殖，提示其可能参与调节妊娠早期山羊子宫的能量代谢平衡。
