College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
Qinghai Provincial Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Qinghai 810008, China.
Food Chem. 2022 Nov 1;393:133351. doi: 10.1016/j.foodchem.2022.133351. Epub 2022 Jun 3.
Antibiotic abuse has caused serious health risks for human beings for long. To address the problem, novel and facile detection techniques are highly desired. Here, an effective multiplex immunochromatographic platform (MICP) with synthesis-free and cost-effective merits is established for simultaneous detection of antibiotics on a single immunochromatographic assay (ICA) strip. Adopting crystal violet (CV) as a signal tag for multiplex ICA allows for direct coupling with multiple antibodies in several minutes. By combining CV and ICA perfectly, this convenient strategy offers improvements in convenience, speed, flexibility, and portability, eventually ensuring the optimized effectiveness of this approach. As a result, the established platform is successfully used to detect streptomycin (STR) and chloramphenicol (CAP) with visual detection mode, and the obtained total recoveries of milk and honey real samples changed from 83.82 to 113.38% with total RSD values of 0.48 to 4.15%.
抗生素滥用长期以来给人类健康带来了严重的风险。为了解决这个问题，人们非常希望开发新型、简单易用的检测技术。在这里，建立了一种具有合成免费和经济高效优点的有效多重免疫层析平台（MICP），可在单个免疫层析分析（ICA）条上同时检测抗生素。采用结晶紫（CV）作为多重 ICA 的信号标记物，可以在几分钟内直接与多种抗体偶联。通过将 CV 和 ICA 完美结合，这种便捷的策略在便利性、速度、灵活性和便携性方面都得到了改进，最终确保了该方法的优化效果。结果，该平台成功地用于以可视化检测模式检测链霉素（STR）和氯霉素（CAP），并将牛奶和蜂蜜实际样品的总回收率从 83.82%提高到 113.38%，总相对标准偏差（RSD）值从 0.48%提高到 4.15%。
