Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Laboratory of Reproductive Biology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark.
Theriogenology. 2022 Aug;188:135-144. doi: 10.1016/j.theriogenology.2022.05.013. Epub 2022 May 25.
Recent in vitro follicle culture (IVFC) studies in caprine have yielded lower maturation rates using late preantral follicles compared to early antral follicles. Thus, research focusing on developing stage-specific customized culture systems able to improve the efficiency of IVFC for late preantral follicles are warranted. This study aimed to compare the morphometric features, estradiol production, and gene expression between early antral caprine follicles produced in vitro and in vivo. In vitro-derived early antral follicles were produced after a 6-day in vitro culture of late preantral follicles, while in vivo-derived early antral follicles were yielded immediately after isolation from the ovaries; antral follicles were, thereafter, cultured for 18 days. In vitro-derived antral follicles were cultured either in a medium developed for preantral follicles (PF medium) or in a medium developed for antral follicles (AF medium). In vivo-derived early antral follicles, on the other hand, were cultured in AF medium (Control treatment). Results demonstrated that in vitro-derived antral follicles cultured in PF medium produced higher estradiol concentration, and m-RNA expression for matrix metalloproteinase-9 (MMP-9), and insulin receptor when compared to both in vitro- and in vivo-derived antral follicles cultured in AF medium. Remarkably, in vitro-derived antral follicles cultured in PF medium had similar MII and oocytes ≥110 μm rates compared with in vivo-derived antral follicles (Control treatment). In conclusion, when cultured in a single and appropriate medium (i.e., PF medium), in vitro-derived early antral follicles had comparable oocyte maturation rates to the in vivo-derived early antral follicles.
最近的山羊体外卵泡培养（IVFC）研究表明，与早期腔前卵泡相比，晚期腔前卵泡的成熟率较低。因此，有必要研究开发针对特定发育阶段的定制培养系统，以提高晚期腔前卵泡 IVFC 的效率。本研究旨在比较体外和体内产生的早期腔前山羊卵泡的形态特征、雌二醇产生和基因表达。体外衍生的早期腔前卵泡是通过对晚期腔前卵泡进行 6 天的体外培养产生的，而体内衍生的早期腔前卵泡则是在从卵巢中分离后立即产生的；此后，将腔前卵泡培养 18 天。体外衍生的腔前卵泡分别在用于腔前卵泡的培养基（PF 培养基）或用于腔前卵泡的培养基（AF 培养基）中培养。另一方面，体内衍生的早期腔前卵泡在 AF 培养基（对照处理）中培养。结果表明，与在 AF 培养基中培养的体外和体内衍生的腔前卵泡相比，在 PF 培养基中培养的体外衍生的腔前卵泡产生的雌二醇浓度更高，基质金属蛋白酶-9（MMP-9）和胰岛素受体的 m-RNA 表达更高。值得注意的是，在 PF 培养基中培养的体外衍生的腔前卵泡的 MII 和直径≥110μm 的卵母细胞率与体内衍生的腔前卵泡（对照处理）相似。总之，在单一且合适的培养基（即 PF 培养基）中培养时，体外衍生的早期腔前卵泡的卵母细胞成熟率与体内衍生的早期腔前卵泡相当。
