College of Life Sciences, Qufu Normal University, Qufu 273165, Shandong Province, China.
College of Life Sciences, Qufu Normal University, Qufu 273165, Shandong Province, China.
Comp Biochem Physiol A Mol Integr Physiol. 2022 Sep;271:111256. doi: 10.1016/j.cbpa.2022.111256. Epub 2022 Jun 8.
Leptin secreted mainly by white adipose tissues (WAT) plays an important role in immune responses. To understand the role of energy status and leptin in immunity, bilateral perigonadal fat pads were removed or sham-removed in male striped hamsters (Cricetulus barabensis). Half of these hamsters were injected with sterile saline, and another half were administrated with exogenous leptin each day, which lasted for 20 days. Fat removal reduced total body fat mass and leptin titers significantly, leptin administration increased leptin levels in the fat removed hamsters to the control levels, but did not affect total body fat mass. Body mass and gross energy intake were not affected by fat removal, leptin supplement or their interaction. Fat removal decreased thymus mass, phytohaemagglutinin (PHA) response at 12 h, and the levels of immunoglobin (Ig) G 5, IgG10, IgM5, IgM10, IL-2, IL-4, and TNF-α, indicating a reduction in fat mass suppressed cellular and humoral immunity and the production of cytokines. However, fat removal had no effect on spleen mass, bacteria killing activity and IFN-γ titers. Leptin supplement increased PHA response at 6 h and 12 h, and the levels of IgG5, IgG10, IL-4, and IFN-γ to the control levels, implying its boosting effects on these parameters. In addition, leptin level was positively correlated with body fat mass, PHA 6 h, 12 h, Ig G10, Ig M5, Ig M10, IL-2, IL-4, and TNF-α. Collectively, these findings implied leptin was a link between energy status and immunity, and leptin mediated the suppressive effects of reduced energy storage on cellular and humoral immunity.
瘦素主要由白色脂肪组织 (WAT) 分泌，在免疫反应中发挥重要作用。为了了解能量状态和瘦素在免疫中的作用，雄性条纹仓鼠 (Cricetulus barabensis) 的双侧性腺周脂肪垫被切除或假切除。这些仓鼠中的一半每天注射无菌生理盐水，另一半每天给予外源性瘦素，持续 20 天。脂肪去除显著降低了总体脂肪量和瘦素滴度，瘦素给药将去除脂肪的仓鼠中的瘦素水平提高至对照水平，但不影响总体脂肪量。脂肪去除、瘦素补充或它们的相互作用均不影响体重和总能量摄入。脂肪去除降低了胸腺质量、植物血球凝集素 (PHA) 12 小时反应以及免疫球蛋白 (Ig) G5、IgG10、IgM5、IgM10、IL-2、IL-4 和 TNF-α的水平，表明脂肪量减少抑制了细胞和体液免疫以及细胞因子的产生。然而，脂肪去除对脾质量、细菌杀伤活性和 IFN-γ滴度没有影响。瘦素补充增加了 PHA 6 小时和 12 小时反应以及 IgG5、IgG10、IL-4 和 IFN-γ的水平至对照水平，表明其对这些参数具有增强作用。此外，瘦素水平与体脂肪量、PHA 6 小时、12 小时、IgG10、IgM5、IgM10、IL-2、IL-4 和 TNF-α呈正相关。总之，这些发现表明瘦素是能量状态和免疫之间的联系，瘦素介导了能量储存减少对细胞和体液免疫的抑制作用。
