Department of Veterinary Medicine, Università degli Studi di Milano, Via dell'Università 6, 26900, Lodi, Italy.
Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Via Balzaretti, 9/11/13, 20133, Milan, Italy.
Sci Rep. 2022 Jun 11;12(1):9665. doi: 10.1038/s41598-022-13400-0.
This study aimed to determine the lipidome of water buffalo milk with intramammary infection (IMI) by non-aureus staphylococci (NAS), also defined as coagulase-negative staphylococci, using an untargeted lipidomic approach. Non-aureus Staphylococci are the most frequently isolated pathogens from dairy water buffalo milk during mastitis. A total of 17 milk samples from quarters affected by NAS-IMI were collected, and the lipidome was determined by liquid chromatography-quadrupole time-of-flight mass spectrometry. The results were compared with the lipidome determined on samples collected from 16 healthy quarters. The study identified 1934 different lipids, which were classified into 15 classes. The abundance of 72 lipids changed in NAS-IMI milk compared to healthy quarters. Significant changes occurred primarily in the class of free fatty acids. The results of this study provided first-time insight into the lipidome of dairy water buffalo milk. Moreover, the present findings provide evidence that NAS-IMI induces changes in water buffalo milk's lipidome.
本研究旨在应用非金黄色葡萄球菌（NAS），即凝固酶阴性葡萄球菌（CNS）的非靶向脂质组学方法，确定患有乳腺炎的水牛乳的脂质组。在乳腺炎期间，CNS 是从奶牛乳中分离出的最常见的病原体。本研究共采集了 17 个受 NAS-IMI 影响的乳区的乳样，通过液相色谱-四极杆飞行时间质谱法来确定脂质组。并将结果与从 16 个健康乳区采集的样本的脂质组进行比较。研究共鉴定出 1934 种不同的脂质，将其分为 15 类。与健康乳区相比，NAS-IMI 乳中的 72 种脂质丰度发生了变化。主要发生在游离脂肪酸这一类。本研究首次提供了关于水牛乳脂质组的见解。此外，本研究结果为 NAS-IMI 引起水牛乳脂质组发生变化提供了证据。
