表面处理和树脂复合水门汀固化方式对成纤维细胞行为的影响。

Influence of surface treatment and curing mode of resin composite cements on fibroblast behavior.

机构信息

Biomaterials and Technology, Department of Reconstructive Dentistry, University Center for Dental Medicine Basel UZB, University of Basel, Mattenstrasse 40, CH-4058, Basel, Switzerland.

出版信息

Head Face Med. 2022 Jun 11;18(1):18. doi: 10.1186/s13005-022-00323-4.

DOI:10.1186/s13005-022-00323-4

PMID:35690829

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9188230/

Abstract

BACKGROUND

Human gingival fibroblast (HGF-1) cells in the connective tissue provide an effective barrier between the alveolar bone and the oral environment. Cement margins of restorations with intrasulcular preparation or cemented implant restorations are in contact with HGF cells. However, it is unknown to what extend the cement surface finish affects the behavior of HGF cells. The purpose of this study was to compare the behavior of HGF-1 cells in contact with two different resin composite cements with three different surface treatments after light-curing and autopolymerization, respectively.

METHODS

Disks of one adhesive (Multilink Automix, Ivoclar Vivadent [MLA]) and one self-adhesive (RelyX Unicem 2 Automix, 3 M [RUN]) resin composite cement were either light-cured or autopolymerized. Specimen surfaces were prepared with the oxygen inhibition layer intact, polished with P2500-grit silicon carbide paper or treated with a scaler. Cells were cultivated on the specimens for 24 h. Viability assay was performed, and cell morphology was examined with scanning electron microscopy. Additionally, roughness parameters of the specimen were analyzed with a 3D laser scanning microscope. Three-way ANOVA was applied to determine the effect of cement material, curing mode and surface treatment (a = 0.05).

RESULTS

Overall, cement material (p = 0.031), curing mode (p = 0.001), and surface treatment (p < 0.001) significantly affected relative cell viability of HGF. The autopolymerized specimen with the oxygen inhibition layer left intact displayed the lowest relative cell viability (MLA 25.7%, RUN 46.6%). Removal of the oxygen inhibition layer with a scaler increased cell viability but also resulted in higher surface roughness values.

CONCLUSIONS

HGF cell viability is affected by the surface treatment and the curing mode. The oxygen inhibition layer is an inhibitory factor for the viability of HGF cells. Autopolymerization enhances the cytotoxic potential of the oxygen inhibition layer.

摘要

背景

结缔组织中的人牙龈成纤维细胞（HGF-1）为牙槽骨和口腔环境之间提供了有效的屏障。具有内表面预备或粘结种植体修复体的修复体的水泥边缘与 HGF 细胞接触。然而，尚不清楚水泥表面光洁度在何种程度上影响 HGF 细胞的行为。本研究的目的是比较两种不同的树脂复合水泥在光固化和自聚合后与三种不同的表面处理接触时 HGF-1 细胞的行为。

方法

将一个粘结剂（Multilink Automix，Ivoclar Vivadent [MLA]）和一个自粘结剂（RelyX Unicem 2 Automix，3M [RUN]）的树脂复合水泥分别用光固化或自聚合处理。将试件表面的氧抑制层完整地制备，用 P2500 粒度碳化硅砂纸抛光或用压片机处理。将细胞培养在试件上 24 小时。进行生存能力测定，并通过扫描电子显微镜观察细胞形态。此外，用三维激光扫描显微镜分析试件的粗糙度参数。用三因素方差分析（a=0.05）确定水泥材料、固化方式和表面处理的影响。