Regenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Fredrik Bajers Vej 3B, 9220, Aalborg, Denmark.
Stem Cell Res Ther. 2022 Jun 11;13(1):249. doi: 10.1186/s13287-022-02928-7.
In pre-clinical studies, human adipose-derived stem cells (hASCs) have shown great promise as a treatment modality for healing of cutaneous wounds. The advantages of hASCs are that they are relatively easy to obtain in large numbers from basic liposuctions, they maintain their characteristics after long-term in vitro culture, and they possess low immunogenicity, which enables the use of hASCs from random donors. It has been hypothesized that hASCs exert their wound healing properties by reducing inflammation, inducing angiogenesis, and promoting fibroblast and keratinocyte growth. Due to the inherent variability associated with the donor-dependent nature of ASC-based products, it appears necessary that the quality of the different products is prospectively certified using a set of most relevant potency assays. In this review, we present an overview of the available methodologies to assess the Mode and the Mechanism of Action of hASCs, specifically in the wound healing scenario. In conclusion, we propose a panel of potential potency assays to include in the future production of ASC-based medicinal products.
在临床前研究中，人脂肪来源的干细胞（hASCs）作为治疗皮肤创伤的一种方法显示出巨大的潜力。hASCs 的优点是它们相对容易从基础吸脂术大量获得，在长期体外培养后保持其特性，并且具有低免疫原性，这使得可以使用来自随机供体的 hASCs。据推测，hASCs 通过减少炎症、诱导血管生成以及促进成纤维细胞和角质细胞生长来发挥其伤口愈合特性。由于与基于 ASC 的产品的供体依赖性相关的固有可变性，似乎有必要使用一组最相关的效力测定法来前瞻性地验证不同产品的质量。在这篇综述中，我们介绍了评估 hASCs 的作用模式和作用机制的现有方法的概述，特别是在伤口愈合的情况下。总之，我们提出了一组潜在的效力测定法，以纳入未来基于 ASC 的药物产品的生产。
