基于深共晶溶剂的三相分配法用于番茄过氧化物酶的纯化：一种替代叔丁醇的有前景的方法。
Deep eutectic solvents-based three-phase partitioning for tomato peroxidase purification: A promising method for substituting t-butanol.
机构信息
Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
出版信息
Food Chem. 2022 Nov 1;393:133379. doi: 10.1016/j.foodchem.2022.133379. Epub 2022 Jun 2.
T-butanol is widely used in three-phase partitioning (TPP). This study used deep eutectic solvents (DESs) to substitute t-butanol for tomato peroxidase (POD) purification. DES-5 (menthol and octanoic acid) was screened as the optimal solvent. The extraction process was optimized using single-factor experiments. Thereafter, the major three factors were optimized by response surface methodology (RSM). When the ammonium sulfate ((NH)SO) concentration was 42%, DES: crude extract (v/v) was 2:1, pH was 5.7, and extraction temperature was 30 °C, the recovery and purification fold reached a maximum of 104.71% and 9.76, respectively. The obtained tomato POD was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which showed that this system could effectively purify POD. Finally, recycling studies indicated the good cycle stability of the DES. This TPP based on DESs is greener and more efficient, indicating that DESs can be good alternative solvents for further applications of TPP.
叔丁醇广泛应用于三相分配（TPP）中。本研究使用深共熔溶剂（DESs）代替叔丁醇来纯化番茄过氧化物酶（POD）。筛选出 DES-5（薄荷醇和辛酸）作为最佳溶剂。通过单因素实验对提取工艺进行了优化。然后，采用响应面法（RSM）对主要的三个因素进行了优化。当硫酸铵（（NH）SO）浓度为 42%、DES：粗提物（v/v）为 2:1、pH 值为 5.7、提取温度为 30°C 时，回收率和纯化倍数分别达到 104.71%和 9.76 的最大值。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析得到的番茄 POD，表明该体系可以有效纯化 POD。最后，循环研究表明 DES 具有良好的循环稳定性。基于 DES 的这种 TPP 更加环保和高效，表明 DES 可以作为进一步应用 TPP 的良好替代溶剂。
Zotero 插件