数字 PCR 在确定 Xpert Xpress SARS-CoV-2 检测试剂盒 E 基因阴性和 N2 基因阳性结果的可靠性中的应用。
Application of digital PCR to determine the reliability of Xpert Xpress SARS-CoV-2 assay with envelope (E) gene negative and nucleocapsid (N2) gene positive results.
机构信息
Department of Microbiology, Prince of Wales Hospital, Hong Kong.
Department of Microbiology, Prince of Wales Hospital, Hong Kong.
出版信息
Diagn Microbiol Infect Dis. 2022 Aug;103(4):115726. doi: 10.1016/j.diagmicrobio.2022.115726. Epub 2022 May 20.
Abstract
This study used digital polymerase chain reaction (dPCR) to determine whether envelope (E) gene-negative and nucleocapsid (N2) gene-positive (E-N+) results obtained with the Cepheid Xpert Xpress SARS-CoV-2 assay are reliable. Using droplet digital PCR results as a reference, 18 of 22 E-N+ samples with a low viral load (81.8%) were identified as true positives.
摘要
本研究使用数字聚合酶链反应（dPCR）来确定使用 Cepheid Xpert Xpress SARS-CoV-2 检测试剂盒获得的包膜（E）基因阴性和核衣壳（N2）基因阳性（E-N+）结果是否可靠。以液滴数字 PCR 结果为参考，22 份低病毒载量的 E-N+样本中有 18 份（81.8%）被确认为真正的阳性。
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