Department of Forensic Science, School of Basic Medical Sciences, Central South University, 410013, Changsha, Hunan, China.
Institute of Forensic Science, Public Security Department of Hunan Province, 410013, Changsha, Hunan, China.
J Forensic Leg Med. 2022 Aug;90:102388. doi: 10.1016/j.jflm.2022.102388. Epub 2022 Jun 7.
Flualprazolam, a new benzodiazepine psychoactive substance recently made available online, and outside the controlled substance list, is often used by criminals for rape and robbery. In this paper, flualprazolam was successfully identified by gas chromatography-mass spectrometry (GC-MS), liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) and nuclear magnetic resonance (NMR). Moreover, LC-Q-TOF-MS analysis method was proposed for the determination of flualprazolam in whole blood using the rabbit perfusion model. After metabolism analysis, a monohydroxylated metabolite 3-hydroxy-flualprazolam was found in the primary mass spectrum of metabolites. Meanwhile, the time effect curve of the flualprazolam in rabbit's blood was explored and the detection window was about 36 h. Moreover, the sensitivity of the established LC-Q-TOF-MS method was investigated with the limit of detection of 0.03 ng/mL. The successful analysis of an actual forensic case with this established method suggests that it might provide a reference method for drug detection or supervision in law enforcement agencies and identification institutions.
氟拉苯达唑，一种最近在网上出现的新型苯并二氮䓬类精神活性物质，且不在受控物质清单内，常被犯罪分子用于强奸和抢劫。本文通过气相色谱-质谱联用（GC-MS）、液相色谱-四极杆飞行时间质谱联用（LC-Q-TOF-MS）和核磁共振（NMR）成功鉴定了氟拉苯达唑。此外，还采用兔灌注模型，提出了 LC-Q-TOF-MS 分析方法，用于检测全血中的氟拉苯达唑。经代谢分析，在代谢物的一级质谱中发现了单羟基代谢物 3-羟基-氟拉苯达唑。同时，还探讨了氟拉苯达唑在兔血中的时效曲线，检测窗口约为 36 小时。此外，还考察了所建立的 LC-Q-TOF-MS 方法的灵敏度，检测限为 0.03ng/mL。利用该方法成功分析了一个实际的法医案例，表明它可能为执法机构和鉴定机构的药物检测或监督提供参考方法。
