Effects of nitrogen and phosphorus availability on cadmium tolerance in the marine diatom Phaeodactylum tricornutum.

Although the influence of major nutrients on metal toxicity in marine phytoplankton has been widely explored, the mechanisms involving the cell surface are poorly understood. Here, the model marine diatom Phaeodactylum tricornutum was cultured under different nitrogen (N), and phosphorus (P) availabilities from the f/2 to the f/20 level in the laboratory; the diatom's accumulation of cadmium (Cd) and the effects of the physical and chemical properties of the cell wall were investigated at the single-cell level. Under higher N and/or P supply at the f/2 level, both the adsorption and uptake of Cd were enhanced in the P. tricornutum cells. The N and P increased the ion-binding sites on the cell surface, causing more negative surface potential and less depolarization of the diatoms' cell walls. Up-regulated transporter genes were detected in those cells with enriched nutrient supply, which could be attributed to the higher Cd uptake. These results strongly indicate that N and P are critical nutrients for frustule-mediated metal accumulation and tolerance in marine diatoms. Our study provides new clues on the nutrient-dependent cell-surface physical and chemical mechanisms involved in metal toxicity in marine diatoms.