在丁酸梭菌中添加磁铁矿加速乳酸盐利用途径以增强生物氢的产生。
Acceleration of lactate-utilizing pathway for enhancing biohydrogen production by magnetite supplementation in Clostridium butyricum.
机构信息
Green and Sustainable Materials R&D Department, Korea Institute of Industrial Technology (KITECH), Cheonan-si 31056, Republic of Korea; School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea.
Green and Sustainable Materials R&D Department, Korea Institute of Industrial Technology (KITECH), Cheonan-si 31056, Republic of Korea.
出版信息
Bioresour Technol. 2022 Sep;359:127448. doi: 10.1016/j.biortech.2022.127448. Epub 2022 Jun 9.
A conductive metal compound can be used as a catalyst for enhancing hydrogen production by dark fermentation. This study aimed to identify mechanisms of enhanced hydrogen production by magnetite supplementation. Experiments were performed with lactate and/or magnetite supplementation to confirm that the lactate-utilizing pathway is the key cause of enhanced hydrogen production. Also, ribonucleic acid sample was collected for monitoring gene regulation under each condition. Hydrogen production was significantly enhanced by approximately 25.6% and 58.9%, respectively, via magnetite alone and with lactate. Moreover, the expression of genes involved in hydrogen production, including pyruvate ferredoxin oxidoreductase, hydrogenase, and ferredoxin, via magnetite alone and with lactate was upregulated by 0.26, 0.71, and 3.50 and 1.06, 2.14, and 1.94 times, respectively.
一种导电金属化合物可用作催化剂，以增强暗发酵制氢。本研究旨在确定磁铁矿补充对提高氢气产量的作用机制。通过添加乳酸盐和/或磁铁矿进行实验，证实了利用乳酸盐的途径是提高氢气产量的关键原因。此外，还收集了核糖核酸样本，以监测每种条件下的基因调控。单独添加磁铁矿和同时添加乳酸盐，可分别将氢气产量提高约 25.6%和 58.9%。此外，单独添加磁铁矿和同时添加乳酸盐可使与氢气生成相关的基因，包括丙酮酸铁氧还蛋白氧化还原酶、氢化酶和铁氧还蛋白的表达分别上调 0.26、0.71 和 3.50 倍和 1.06、2.14 和 1.94 倍。
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