da Silva Livia Maria Alves Valentim, Cintra Luciano Tavares Angelo, de Alcântara Sibele, Machado Nathália Evelyn da Silva, Benetti Francine, Ervolino Edilson, Briso André Luiz Fraga
Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry Araçatuba, José Bonifácio, Vila Mendonça, Araçatuba, São Paulo 1193, Brazil.
Department of Restorative Dentistry, School of Dentistry, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
Photodiagnosis Photodyn Ther. 2022 Sep;39:102959. doi: 10.1016/j.pdpdt.2022.102959. Epub 2022 Jun 9.
To evaluate the influence of violet LED, associated or not with a 17.5% hydrogen peroxide (HP) bleaching gel, on inflammation, mineralization in pulp tissue, and collagen fiber maturation in dentin and pulp tissue.
The maxillary molars of eighty Wistar rats were distributed into four groups (n = 10): CONT - without treatment; HP - 30 min application of 17.5% HP; LED - 20 min application of violet LED; and HP+LED - application of PH and violet LED. Rats were euthanized and jaws were processed for histologic and immunohistochemical evaluation (IL-17, IL-23, and osteocalcin) and picrosirius red immediately after (T0), and at 7 (T1), 15 (T2), and 30 days (T3) post-treatment, with Wilcoxon, Mann-Whitney, paired T-test, and T-test (α = 0.05).
HP and HP+LED presented necrosis and severe inflammatory infiltrate. When compared to CONT group, LED presented severe osteocalcin (OCN) immunostaining in T2 and less immature fibers in T2 and T3.
The violet LED caused no severe damage to the pulp tissue, increased IL-17 and IL-23 expression in T0 when associated with HP, and had no influence on pulp tissue mineralization, besides accelerating the maturation of collagen fibers of dentin.
Violet LED therapy induced no inflammation in the pulp tissue of rats and played no role in pulp tissue fibrosis, besides accelerating the maturation of dentin collagen fibers.
评估紫色发光二极管（LED）联合或不联合17.5%过氧化氢（HP）漂白凝胶对牙髓组织炎症、矿化以及牙本质和牙髓组织中胶原纤维成熟的影响。
将80只Wistar大鼠的上颌磨牙分为四组（n = 10）：对照组（CONT）——未治疗；HP组——应用17.5% HP 30分钟；LED组——应用紫色LED 20分钟；HP + LED组——应用HP和紫色LED。大鼠处死后，立即（T0）以及在治疗后7天（T1）、15天（T2）和30天（T3）对颌骨进行组织学和免疫组织化学评估（白细胞介素-17、白细胞介素-23和骨钙素）以及苦味酸天狼星红染色，采用Wilcoxon检验、Mann-Whitney检验、配对t检验和t检验（α = 0.05）。
HP组和HP + LED组出现坏死和严重炎症浸润。与对照组相比，LED组在T2时骨钙素（OCN）免疫染色严重，在T2和T3时未成熟纤维较少。
紫色LED对牙髓组织未造成严重损伤，与HP联合使用时在T0时增加白细胞介素-17和白细胞介素-23的表达，对牙髓组织矿化无影响，此外还加速了牙本质胶原纤维的成熟。
紫色LED疗法在大鼠牙髓组织中未诱发炎症，在牙髓组织纤维化中不起作用，此外还加速了牙本质胶原纤维成熟。
