[Association between periodontitis and mild cognitive impairment: a clinical pilot study].

To evaluate the association between periodontitis and mild cognitive impairment (MCI), and explore the potential local oral risk factors for MCI. The study included 70 middle-aged and elderly subjects (44 females and 26 males) with periodontal disease who were first diagnosed by the Department of Periodontology or referred by the Department of Geriatrics in Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from January 2021 to January 2022. In this study, the control group consisted of periodontal disease patients without cognitive impairment, and the case group (MCI group) consisted of those diagnosed with MCI referred by the geriatrics specialists. Full-mouth periodontal examinations of all subjects were performed and periodontal indicators were recorded by periodontists, while digital panoramic radiographs were taken. The severity of periodontitis was defined according to the 1999 classification, and the staging and grading of periodontitis were defined according to the 2018 American Academy of Periodontology and European Federation of Periodontology classification. The mini-mental state examination scale was chosen by geriatricians to evaluate the cognitive function of the included subjects. The cubital venous blood was drawn to detect the expression levels of inflammatory factors such as hypersensitive C-reactive protein (hs-CRP), interleukin (IL)-1β, IL-6 and tumor necrosis factor-α(TNF-α) in serum. Independent-samples test and test were used to analyze the differences in population factors, periodontal-related indexes and serum inflammatory factors between the two groups (α=0.05). Odds ratios () for MCI according to the severity of periodontitis and main periodontal clinical indexes were calculated by binary Logistic analysis. Thirty-nine subjects were included in the control group and thirty-one in the MCI group. The age of the study population was (58.3±6.2) years (range: 45-70 years). The comparison between two groups showed that the control group was with higher educational background (χ²=9.45, 0.024) and 2.6 years younger than the MCI group [(57.1±6.0) years . (59.7±6.3) years, -1.24, 0.082]. The number and proportion of moderate to severe periodontitis in control group were significantly lower compared to those in MCI group (17 cases with 43.6% . 23 cases with 74.2%, χ²=6.61, 0.010), and the of moderate to severe periodontitis adjusted by age and educational background was 3.00 (95: 1.01-8.86, =0.048). Compared with the grading (χ²=5.56, 0.062) of periodontitis, staging had a greater impact on MCI (χ²=7.69, 0.041), moreover the proportion of MCI in stage Ⅰ grade A periodontitis was significantly lower than any other type of periodontitis (χ²=13.86, 0.036). In addition, less presence of deep periodontal pockets [probing depth (PD)≥6 mm] (17.9% . 41.9%, χ²=4.87, 0.027), fewer number of PD≥4 mm (6.48±6.70 . 11.03±8.91, -2.44, =0.017), lower plaque index (1.42±0.56 . 1.68±0.57, -1.91, 0.059) and gingival index (1.68±0.29 . 1.96±0.30, -3.93, 0.001) were in the control group than in the MCI group. However, there were no significant differences between the two groups in the levels of serum inflammatory factors, such as hs-CRP, IL-1β, IL-6 and TNF-α (0.05). It appears a strong correlation between moderate to severe periodontitis and the incidence of MCI in middle-aged and elderly people. Moreover, deep and increased number of periodontal pockets, poor oral hygiene, and severe gingival inflammation can be potentially associated risk factors for MCI.