Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Tokyo, Japan.
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China.
J Exp Zool A Ecol Integr Physiol. 2022 Aug;337(7):760-767. doi: 10.1002/jez.2633. Epub 2022 Jun 12.
Sugar cane extract (SCE) is the end product of glucose, fructose, and sucrose elimination in molasses. SCE has various biological effects, such as anti-inflammation and antioxidation, and it is commonly found in animal feed. The present research is aimed at investigating the reproductive endocrine influence of SCE in male Japanese quails (Coturnix japonica) by feeding SCE containing food. In addition, in vitro Leydig cell culture was conducted to clarify the mechanism of SCE's influence. Our results showed that SCE feed extended the latency to the first neck grab, decreased male quail testis and epididymis weights, cloaca gland size, and reduced serum testosterone concentrations. Steroidogenic enzymes 3βHSD, 17βHSD, P450c17, and P450scc gene expression in the testis were decreased in the SCE groups. Western blot analysis showed decreased 3βHSD in the testis after feeding SCE. Isolated testicular interstitial cells cultured with SCE and ovine-LH suppressed testosterone secretion and 3βHSD gene expression. In conclusion, SCE as a feed additive has an impact on the sexual behavior and reproductive function of male Japanese quail, with the suppression of steroidogenesis in the Leydig cell. Our results may provide beneficial information to the livestock management and the poultry industry.
甘蔗提取物（SCE）是糖蜜中葡萄糖、果糖和蔗糖去除的最终产物。SCE 具有多种生物学作用，如抗炎和抗氧化作用，常用于动物饲料。本研究旨在通过喂食含有 SCE 的食物来研究 SCE 对雄性日本鹌鹑（Coturnix japonica）生殖内分泌的影响。此外，还进行了体外 Leydig 细胞培养，以阐明 SCE 影响的机制。我们的结果表明，SCE 饲料延长了首次颈部抓握的潜伏期，降低了雄性鹌鹑睾丸和附睪的重量、肛殖腔腺的大小，并降低了血清睾酮浓度。SCE 组睾丸中的 3βHSD、17βHSD、P450c17 和 P450scc 基因的类固醇生成酶表达降低。Western blot 分析表明，喂食 SCE 后睾丸中的 3βHSD 减少。用 SCE 和绵羊-LH 培养的分离睾丸间质细胞抑制了睾酮分泌和 3βHSD 基因表达。总之，SCE 作为饲料添加剂对雄性日本鹌鹑的性行为和生殖功能有影响，抑制了 Leydig 细胞中的类固醇生成。我们的结果可能为畜牧业管理和家禽业提供有益信息。
