University of Copenhagen, Faculty of Science, CPSC, Department of Plant and Environmental Sciences, Frederiksberg C Denmark.
Universidad Politécnica de Madrid, Campus de Montegancedo, Centre for Plant Biotechnology and Genomics (UPM-INIA), Madrid, Spain.
Plant Signal Behav. 2022 Dec 31;17(1):2084278. doi: 10.1080/15592324.2022.2084278.
Plant innate immunity toward cell-wall penetrating filamentous pathogens relies on the conserved SYP12 clade of secretory syntaxins. In , the two closely related SYP12 clade members, PEN1 and SYP122, play an overlapping role in this general immunity, which can be complemented by two SYP12 clade members from (MpSYP12A and MpSYP12B). However, in addition to the conserved SYP12 clade function, PEN1 alone mediates pre-invasive immunity toward powdery mildew fungi, which likely reflects a specialization of its functionality. Here, we show that the PEN1-specific specialization in immunity correlates with a continuous BFA-sensitive recycling and the ability to accumulate strongly at the growing cell plate. This contrasts with the behavior of SYP122, MpSYP12A, and MpSYP12B, all being more stable at the plasma membrane. We suggest that the highly mobile SYP12 specialization observed for PEN1 is required for a fast pre-invasive immune response to resist attack from powdery mildew fungi.
植物对细胞壁穿透丝状病原体的先天免疫依赖于保守的 SYP12 分泌融合蛋白家族。在 中，两个密切相关的 SYP12 家族成员 PEN1 和 SYP122 在这种普遍免疫中发挥重叠作用，这可以由来自 （MpSYP12A 和 MpSYP12B）的两个 SYP12 家族成员来补充。然而，除了保守的 SYP12 家族功能外，PEN1 单独介导对粉孢菌的侵入前免疫，这可能反映了其功能的专业化。在这里，我们表明 PEN1 在免疫中的特异性专业化与持续的 BFA 敏感性回收以及在生长的细胞板处强烈积累的能力相关。这与 SYP122、MpSYP12A 和 MpSYP12B 的行为形成对比，它们在质膜上更稳定。我们认为，PEN1 观察到的高度移动的 SYP12 专业化是抵抗粉孢菌攻击所需的快速侵入前免疫反应所必需的。
