Shi Yumei, Luo Changxin, Xiang Yun, Qian Dong
Ministry of Education (MOE) Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.
Front Plant Sci. 2023 Feb 10;14:1120841. doi: 10.3389/fpls.2023.1120841. eCollection 2023.
Cell plates are transient structures formed by the fusion of vesicles at the center of the dividing plane; furthermore, these are precursors to new cell walls and are essential for cytokinesis. Cell plate formation requires a highly coordinated process of cytoskeletal rearrangement, vesicle accumulation and fusion, and membrane maturation. Tethering factors have been shown to interact with the Ras superfamily of small GTP binding proteins (Rab GTPases) and soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), which are essential for cell plate formation during cytokinesis and are fundamental for maintaining normal plant growth and development. In , members of the Rab GTPases, tethers, and SNAREs are localized in cell plates, and mutations in the genes encoding these proteins result in typical cytokinesis-defective phenotypes, such as the formation of abnormal cell plates, multinucleated cells, and incomplete cell walls. This review highlights recent findings on vesicle trafficking during cell plate formation mediated by Rab GTPases, tethers, and SNAREs.
细胞板是由囊泡在分裂平面中央融合形成的临时性结构；此外，这些结构是新细胞壁的前体，对胞质分裂至关重要。细胞板的形成需要细胞骨架重排、囊泡积累与融合以及膜成熟等高度协调的过程。衔接因子已被证明可与小GTP结合蛋白（Rab GTP酶）的Ras超家族以及可溶性N - 乙基马来酰亚胺敏感因子附着蛋白受体（SNAREs）相互作用，这些对于胞质分裂期间的细胞板形成至关重要，并且对于维持植物正常生长发育至关重要。在植物中，Rab GTP酶、衔接因子和SNAREs的成员定位于细胞板，编码这些蛋白质的基因突变会导致典型的胞质分裂缺陷表型，如异常细胞板、多核细胞和不完整细胞壁的形成。本综述重点介绍了由Rab GTP酶、衔接因子和SNAREs介导的细胞板形成过程中囊泡运输的最新研究结果。
