Oda K, Hirose S, Takami N, Misumi Y, Takatsuki A, Ikehara Y
FEBS Lett. 1987 Apr 6;214(1):135-8. doi: 10.1016/0014-5793(87)80028-5.
The effects of brefeldin A on intracellular transport and posttranslational modification of complement C3 (C3) were studied in primary culture of rat hepatocytes. In the control culture C3 was synthesized as a precursor (pro-C3), which was processed to the mature form with alpha- and beta-subunits before its discharge into the medium. In the presence of brefeldin A the secretion of C3 was strongly blocked, resulting in accumulation of pro-C3. However, after a prolonged interval the mature form of C3 was finally secreted. The results indicate that brefeldin A impedes translocation of pro-C3 to the Golgi complex where pro-C3 is converted to the mature form, but not its proteolytic processing, in contrast to the effects of monensin and weakly basic amines.
在大鼠肝细胞原代培养中研究了布雷菲德菌素A对补体C3（C3）细胞内转运和翻译后修饰的影响。在对照培养中，C3以前体（pro-C3）形式合成，在释放到培养基之前被加工成具有α和β亚基的成熟形式。在布雷菲德菌素A存在下，C3的分泌被强烈阻断，导致pro-C3积累。然而，经过较长时间间隔后，C3的成熟形式最终被分泌。结果表明，与莫能菌素和弱碱性胺的作用相反，布雷菲德菌素A阻碍pro-C3转运至高尔基体复合物（pro-C3在高尔基体复合物中转化为成熟形式），但不影响其蛋白水解加工。
