Recombinetics, Inc., St. Paul, MN, USA.
Comparative Biology & Experimental Medicine, Faculty of Veterinary Medicine Biochemistry & Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2022;2495:245-258. doi: 10.1007/978-1-0716-2301-5_13.
As the genetic mutations driving human disease are identified, there is an increasing need for a biomedical model that can accurately represent the disease of interest and provide a platform for potential therapeutic testing. Pigs are a better model for human disease than rodents because of their genetic and physiological similarities to humans. However, current methods to generate porcine models are both technically challenging and expensive. Germline genetic modification through gene edited spermatogonia provides an effective alternative to how these models are developed. Here, we report an improved technique of gene editing in spermatogonia of pigs using CRISPR-Cas9 to generate different edits that reflect the genotypes of human diseases.
随着导致人类疾病的基因突变被识别，人们越来越需要一种能够准确代表相关疾病的生物医学模型，并为潜在的治疗测试提供平台。猪比啮齿动物更能模拟人类疾病，因为它们在遗传和生理上与人类相似。然而，目前生成猪模型的方法既具有技术挑战性，又非常昂贵。通过基因编辑精原细胞进行种系遗传修饰为开发这些模型提供了一种有效的替代方法。在这里，我们报告了一种使用 CRISPR-Cas9 在猪精原细胞中进行基因编辑的改进技术，以产生反映人类疾病基因型的不同编辑。
