Dong Zhenming, Liang Wenfang, Ren Hong, Zhang Yuetao, Wang Hui, Wang Yu
School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 15;279:121485. doi: 10.1016/j.saa.2022.121485. Epub 2022 Jun 7.
Cyanide is a highly toxic substance, and the detection of cyanide in the environment and food samples is critical to public health care. Herein, we rationally designed a mitochondria-targeted near-infrared fluorescent probe BTC for ratiometric monitoring of CN in water, food, living cells, and zebrafish. BTC exhibits a remarkable colorimetric ratiometric fluorescence response to CN with high selectivity, low detection limit (54.3 nM), and large Stokes shift. The cyanide sensing mechanism was demonstrated by NMR and ESI-MS analysis and density functional theory (DFT). More importantly, BTC was used for efficient naked-eye colorimetric detection of CN in sprouting potatoes, almonds, and ginkgo fruit samples. Further, the BTC is capable of situ tracking and imaging cyanide in mitochondria of SMMC-7721 cells and in zebrafish via dual emission channels, and was prepared into a kit for convenient and visual on-site sensing of cyanide in food samples.
氰化物是一种剧毒物质，环境和食品样品中氰化物的检测对公共卫生保健至关重要。在此，我们合理设计了一种线粒体靶向近红外荧光探针BTC，用于对水、食品、活细胞和斑马鱼中的CN进行比率监测。BTC对CN表现出显著的比色比率荧光响应，具有高选择性、低检测限（54.3 nM）和大斯托克斯位移。通过核磁共振（NMR）、电喷雾电离质谱（ESI-MS）分析和密度泛函理论（DFT）证明了氰化物传感机制。更重要的是，BTC用于发芽土豆、杏仁和银杏果样品中CN的高效肉眼比色检测。此外，BTC能够通过双发射通道对SMMC-7721细胞线粒体和斑马鱼中的氰化物进行原位跟踪和成像，并制成试剂盒，用于方便、直观地现场检测食品样品中的氰化物。
