College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Engineering Research Institute of Conservation, Utilization of Natural Bioresources, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
J Ethnopharmacol. 2022 Sep 15;295:115455. doi: 10.1016/j.jep.2022.115455. Epub 2022 Jun 11.
Euscaphis konishii Hayata is a traditional medicinal plant in China, and its leaves are usually used to make dishes for hepatic or gastrointestinal issues by Chinese She nationality. Pharmacological analysis showed that E. konishii leaves contain high levels of flavonoids and chromones with favorable anti-hepatoma effect.
The extract from E. konishii leaves was detected to evaluate its chemical composition, and the alcoholic liver injury mice model was adopted to elucidate its hepatoprotective effects.
The total leaf extract from E. konishii was separated by polyamide column to get the flavonoid and chromone-rich extract (FCE). Single compounds from FCE was purified by gel and Sephadex LH-20 chromatography and analyzed by nuclear magnetic resonance (NMR). The chemical component of FCE was confirmed and quantified by HPLC-MS. The OH·, O, DPPH and ABTS free radical assays were adopted to estimate the antioxidant activity of FCE in vitro. The alcohol-fed model mice were established to assess the hepatoprotective capacity of FCE in vivo, through biochemical determination, histopathological analysis, mitochondrial function measurement, quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) detection and Western blot determination.
8 flavonoids and 2 chromones were recognized in the FCEextract by both NMR and HPLC-MS. FCE represented strong free radicals scavenging activity in vitro. With oral administration, FCE (50, 100 and 200 mg/kg) dose-dependently decreased the serum levels of alanine aminotransferase (ALT), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in alcohol-fed mice. FCE gradually reduced the malondialdehyde (MDA) content, increased the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the alcohol-treated liver tissues. FCE also alleviated the hepatic inflammation, inhibited the hepatocyte apoptosis and lessened the alcohol-induced histological alteration and lipid accumulation in the liver tissues. FCE administration inhibited the overexpression of endoplasmic reticulum (ER) chaperones signaling and unfolded protein response (UPR) pathways to defense the ER-induced apoptosis. Pretreatment with FCE also restored the mitochondrial membrane potentials andadenosine triphosphate (ATP) levels, which in turn suppressed the Cytochrome C release and mitochondria-induced apoptosis.
FCE conferred great protection against alcoholic liver injury, which might be associated with its viability through suppressing reactive oxygen species (ROS) stress and hepatocyte apoptosis.
中国的嘉兰是一种传统药用植物，中国的畲族通常用其叶子来制作治疗肝脏或胃肠道问题的菜肴。药理分析表明，嘉兰叶含有高水平的具有良好抗肝癌作用的类黄酮和色酮。
检测嘉兰叶提取物，以评估其化学成分，并采用酒精性肝损伤小鼠模型阐明其保肝作用。
采用聚酰胺柱分离嘉兰叶总提取物，得到富含类黄酮和色酮的提取物（FCE）。采用凝胶和 Sephadex LH-20 层析从 FCE 中分离出单体化合物，并通过核磁共振（NMR）进行分析。采用 HPLC-MS 确定和定量 FCE 的化学成分。采用 OH·、O、DPPH 和 ABTS 自由基测定法评估 FCE 的体外抗氧化活性。通过生化测定、组织病理学分析、线粒体功能测定、定量实时聚合酶链反应（qRT-PCR）检测和 Western blot 测定，在体内评估 FCE 对酒精喂养模型小鼠的保护作用。
通过 NMR 和 HPLC-MS 鉴定 FCE 提取物中含有 8 种类黄酮和 2 种色酮。FCE 体外具有很强的自由基清除活性。口服给药时，FCE（50、100 和 200mg/kg）剂量依赖性地降低酒精喂养小鼠血清丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）和天冬氨酸氨基转移酶（AST）水平。FCE 逐渐降低丙二醛（MDA）含量，增加酒精处理肝组织中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性。FCE 还减轻了肝组织中的炎症、抑制了肝细胞凋亡，并减轻了酒精引起的组织学改变和脂质积累。FCE 给药抑制内质网（ER）伴侣信号和未折叠蛋白反应（UPR）途径的过度表达，以防御 ER 诱导的细胞凋亡。FCE 预处理还恢复了线粒体膜电位和三磷酸腺苷（ATP）水平，从而抑制细胞色素 C 释放和线粒体诱导的细胞凋亡。
FCE 对酒精性肝损伤具有很好的保护作用，这可能与其通过抑制活性氧（ROS）应激和肝细胞凋亡来维持细胞活力有关。
