Neve R L, Stewart G D, Newcomb P, Van Keuren M L, Patterson D, Drabkin H A, Kurnit D M
Gene. 1986;49(3):361-9. doi: 10.1016/0378-1119(86)90372-0.
We have employed two strategies to isolate random cDNA clones encoded by chromosome 21. In the first approach, a cDNA library representing expressed genes of WA17, a mouse-human somatic cell hybrid carrying chromosome 21 as its sole human chromosome, was screened with total human DNA to identify human chromosome 21-specific cDNAs. The second approach utilized previously characterized single-copy genomic fragments from chromosome 21 as probes to retrieve homologous coding sequences from a human fetal brain cDNA library. Six cDNA clones on chromosome 21 were obtained in this manner. Two were localized to the proximal long arm of chromosome 21, two to the distal portion of the long arm, and one to the region of 21q22 implicated in the pathology of Down syndrome.
我们采用了两种策略来分离由21号染色体编码的随机cDNA克隆。在第一种方法中，用总人类DNA筛选一个代表WA17（一种以21号染色体作为其唯一人类染色体的小鼠-人类体细胞杂种）表达基因的cDNA文库，以鉴定21号染色体特异性cDNA。第二种方法利用先前鉴定的来自21号染色体的单拷贝基因组片段作为探针，从人类胎儿脑cDNA文库中检索同源编码序列。通过这种方式获得了21号染色体上的6个cDNA克隆。其中两个定位于21号染色体长臂近端，两个定位于长臂远端，还有一个定位于与唐氏综合征病理相关的21q22区域。
