Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan 48513, Republic of Korea.
Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea.
Anal Methods. 2022 Jul 7;14(26):2569-2577. doi: 10.1039/d2ay00554a.
A paper-based device patterned with a carbon-black-poly(dimethylsiloxane) (PDMS) mixture is developed as a heating platform for nucleic-acid amplification tests. The photothermal effect of carbon black under 808 nm laser irradiation is used to conduct loop-mediated isothermal amplification (LAMP) to detect () O157:H7, a foodborne pathogen. We characterize the heat generation of carbon black by changing its concentration and the hardness of PDMS. Then, we optimize the minimum laser power required to perform LAMP. The proposed paper-based device requires less than 15 min to perform LAMP, and the result can be confirmed based on the color change observed by the naked eye. The gene of O157:H7 is specifically amplified, with a detection limit of 10 CFU mL. Amplification is also performed by using a laboratory-made laser-diode device, which consumes only 2 W h during its operation. The low cost, disposability, and easy fabrication of the paper-based device make it a powerful tool for point-of-care testing.
一种基于纸张的器件，其表面图案为炭黑-聚二甲基硅氧烷（PDMS）混合物，被开发为用于核酸扩增测试的加热平台。炭黑在 808nm 激光辐照下的光热效应被用于进行环介导等温扩增（LAMP）以检测食源性致病菌 () O157:H7。我们通过改变炭黑的浓度和 PDMS 的硬度来表征炭黑的产热情况。然后，我们优化了进行 LAMP 所需的最小激光功率。该基于纸张的器件进行 LAMP 所需的时间少于 15 分钟，并且可以通过肉眼观察到的颜色变化来确认结果。可以特异性扩增 O157:H7 的 基因，检测限为 10 CFU mL。还使用实验室自制的激光二极管设备进行了扩增，其运行过程中仅消耗 2 Wh 的能量。基于纸张的器件成本低、可一次性使用且易于制造，使其成为即时检测的有力工具。
