Department of Laboratory, Yantaishan Hospital, Yantai City, ShanDong Province, 264000, China
Department of Laboratory, Weihai Stomatological Hospital, Weihai City, ShanDong Province, 264200, China
New Microbiol. 2022 Apr;45(2):130-137.
We explore the association of Malassezia and IL-23/IL-17 axis in the skin lesions of patients with Psoriasis. From October 2018 to October 2020, 202 psoriasis patients were hospitalized in the dermatology department of Yantaishan hospital. The patients' skin lesions were collected, and Malassezia-specific mRNA in the skin lesions was determined. The patients were subdivided into Malassezia high and low distribution groups as per the Malassezia-specific mRNA results. Psoriasis Area and Severity Index (PASI) scores between the two groups were performed. LL-37, IL-23, IL-17A, and tumor necrosis factor α (TNF-α) expression in the skin lesions of the two groups were determined. Malassezia mRNA and the correlation of LL-37 with inflammatory factors TNF-α, IL-23, and IL-17A were determined. The relevance of inflammatory factors, Malassezia infection, and LL-37 content with PASI score were studied. The Malassezia high distribution group was treated with etoconazole, and the effects of treatment on the PASI score, IL-23, TNF-α, and IL-17A were determined. The PASI score, neutrophil, eosinophil, and peripheral blood white blood cell counts, and lgG in the Malassezia high distribution group were significantly higher than in the low distribution group (P<0.05). IL-23, LL-37, TNF-α, and IL-17A levels in the Malassezia high distribution group were significantly higher than in the low distribution group (P<0.05). Malassezia and LL-37 levels had a moderate positive correlation (R=0.5009, P<0.0001). Malassezia and LL-37, IL-17A, TNF-a, and IL- 23 correlated positively. Malassezia, IL-17A, LL37, TNF-a, and IL-23 correlated positively with the PASI score of Psoriasis. Ketoconazole therapy inhibited the PASI score, IL-23, TNF-a, and IL-17A expressions in patients. Malassezia enhances the progression of Psoriasis through the aberrant activation of the IL-23/IL-17 axis.
我们探讨马拉色菌与白细胞介素 23/17 轴在银屑病患者皮损中的相关性。2018 年 10 月至 2020 年 10 月，202 例银屑病患者在烟台山医院皮肤科住院。收集患者皮损，检测皮损中马拉色菌特异性 mRNA。根据马拉色菌特异性 mRNA 结果将患者分为马拉色菌高、低分布组。对两组患者进行银屑病面积和严重程度指数（PASI）评分。检测两组患者皮损中 LL-37、白细胞介素 23（IL-23）、白细胞介素 17A（IL-17A）和肿瘤坏死因子-α（TNF-α）的表达。分析皮损中马拉色菌 mRNA 与炎症因子 TNF-α、IL-23、IL-17A 的相关性，以及炎症因子、马拉色菌感染和 LL-37 含量与 PASI 评分的相关性。研究了炎症因子、马拉色菌感染、LL-37 含量与 PASI 评分的相关性。用酮康唑治疗马拉色菌高分布组，检测治疗前后 PASI 评分、IL-23、TNF-α、IL-17A 的变化。马拉色菌高分布组 PASI 评分、中性粒细胞、嗜酸性粒细胞、外周血白细胞计数、lgG 均显著高于低分布组（P<0.05）。马拉色菌高分布组 IL-23、LL-37、TNF-α、IL-17A 水平显著高于低分布组（P<0.05）。马拉色菌与 LL-37 水平呈中度正相关（R=0.5009，P<0.0001）。马拉色菌与 LL-37、IL-17A、TNF-a、IL-23 呈正相关。马拉色菌、IL-17A、LL37、TNF-a、IL-23 与银屑病 PASI 评分呈正相关。酮康唑治疗抑制了患者的 PASI 评分、IL-23、TNF-a 和 IL-17A 的表达。马拉色菌通过异常激活白细胞介素 23/17 轴促进银屑病的进展。
