Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China.
Talanta. 2022 Nov 1;249:123664. doi: 10.1016/j.talanta.2022.123664. Epub 2022 Jun 9.
Cobalt oxyhydroxide nanosheets (CoOOH) with peroxidase-like activity provide a promising probe for acetylcholinesterase (AChE) sensing through a ratiometric fluorescence strategy. Fluorescence of silicon quantum dots (SiQDs) at 457 nm was quenched by CoOOH on account of inner-filter effect (IFE). Meanwhile, the nonfluorescent o-phenylenediamine (OPD) was catalytically oxidized to 2,3-diaminophenazine (oxOPD) by CoOOH nanosheets with emission at 572 nm. The acetylcholine (ATCh) was catalytically hydrolyzed by AChE to enzymatic thiocholine (TCh), which decomposed CoOOH to Co, recovered the fluorescence of SiQDs and reduced the emission of oxOPD. Fluorescence ratio at F/F serves as signal output for AChE detection within 5 × 10-0.05 and 0.05-10 U mL, with a limit of detection (LOD) of 3.2 × 10 U mL. The sensing strategy was applied for AChE assay in human blood and erythrocyte.
钴基纳米片（CoOOH）具有过氧化物酶样活性，通过比率荧光策略为乙酰胆碱酯酶（AChE）传感提供了一种很有前途的探针。由于内滤效应（IFE），CoOOH 使硅量子点（SiQDs）在 457nm 处的荧光猝灭。同时，CoOOH 纳米片将非荧光邻苯二胺（OPD）催化氧化为发射波长为 572nm 的 2,3-二氨基吩嗪（oxOPD）。乙酰胆碱（ATCh）被 AChE 催化水解生成酶促硫代胆碱（TCh），TCh 分解 CoOOH 为 Co，恢复 SiQDs 的荧光并降低 oxOPD 的发射。荧光比 F/F 作为 AChE 检测的信号输出，检测范围为 5×10-0.05 至 0.05-10 U·mL-1，检测限（LOD）为 3.2×10 U·mL-1。该传感策略应用于人血和红细胞中的 AChE 测定。
