School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China; Beijing Key Laboratory on Resource-oriented Treatment of Industrial Pollutants, Beijing, 100083, China.
School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing, 100083, China; State Key Laboratory of Mineral Processing, Beijing, 102628, China; Beijing Key Laboratory on Resource-oriented Treatment of Industrial Pollutants, Beijing, 100083, China.
Chemosphere. 2022 Oct;304:135319. doi: 10.1016/j.chemosphere.2022.135319. Epub 2022 Jun 11.
Polycyclic aromatic hydrocarbons and heavy metals are typical pollutants in the non-ferrous metal smelting industry. The combination of biodegradation and biomineralization has great development potential for co-contamination removal as an environmentally friendly method. Pyrene (Pyr) and cadmium (Cd) were regarded as model pollutants of co-contamination in this study. A bifunctional bacterial community named Ycp was screened from a non-ferrous smelting slag field soil. The 16S rRNA gene high throughput sequencing analysis showed that Enterobacter was the dominant genus (99.1%). Ycp had adaptability under a wide range of environmental conditions (pH 3-9, salinity 0-10 g L NaCl, Pyr concentration 0-50 mg L, Cd concentration 0-100 mg L), and the removal rate of Pyr and Cd reached 41.8%-76.9%, 82.8%-98.8%, respectively. It was found that compound carbon sources had promoting effect on the removal of Pyr and Cd, with the maximum removal rate of 88.3% and 98.0%. According to the degradation products of Pyr by LC-MS analysis and the mineralized products of Cd by XRD and SEM-EDS analysis, the mechanism of Ycp for co-contamination remediation was: Ycp biodegraded Pyr through salicylic acid and phthalic acid metabolic pathways, and biomineralized Cd into CdCO through microbially induced carbonate precipitation. This study provided a basis for microbial remediation of co-contamination.
多环芳烃和重金属是有色金属冶炼行业的典型污染物。生物降解与生物矿化相结合作为一种环保方法，对共污染去除具有很大的发展潜力。本研究以芘（Pyr）和镉（Cd）为共污染模式污染物。从有色金属冶炼渣场土壤中筛选出一种双功能细菌群落，命名为 Ycp。16S rRNA 基因高通量测序分析表明，肠杆菌属（Enterobacter）是优势属（99.1%）。Ycp 能在广泛的环境条件（pH 值 3-9、盐度 0-10 g L NaCl、Pyr 浓度 0-50 mg L、Cd 浓度 0-100 mg L）下适应，Pyr 和 Cd 的去除率分别达到 41.8%-76.9%和 82.8%-98.8%。研究发现，复合碳源对 Pyr 和 Cd 的去除有促进作用，最大去除率分别为 88.3%和 98.0%。根据 LC-MS 分析 Pyr 的降解产物和 XRD、SEM-EDS 分析 Cd 的矿化产物，Ycp 对共污染修复的作用机制为：Ycp 通过水杨酸和邻苯二甲酸代谢途径生物降解 Pyr，并通过微生物诱导碳酸盐沉淀将 Cd 矿化为 CdCO。本研究为共污染微生物修复提供了依据。
