College of Food and Health, Zhejiang A & F University, # 666, Wusu Street, Lin'an District, Hangzhou, Zhejiang province 311300, China.
New South Wales Department of Primary Industries, Ourimbah, New South Wales 2258, Australia.
J Agric Food Chem. 2022 Jun 15;70(23):7258-7266. doi: 10.1021/acs.jafc.2c01220. Epub 2022 Jun 7.
-induced quality deterioration in stored sweet potato is poorly characterized and understood. This study examined the effects of infection in Xinxiang sweet potato roots during storage. The results showed that while there were no external symptoms following infection, upon cutting the roots, the cut surface of the infected root rapidly turned black, whereas the untreated control roots remained unaffected. The metabolites and transcriptive differences between -infected and control sweet potato roots were investigated with high-performance liquid chromatography, metabolomic analysis, and an Illumina Novaseq platform. The results showed that levels of the toxic ipomeamarone accumulated as high as 2.36 mg/kg DW in tissue after inoculation and 6 days storage at 28 °C, where the control tissue sample did not accumulate any ipomeamarone. Metabolomic analysis showed that isochlorogenic acid and l-tyrosine significantly increased in the infected tissue and associated with the darkening cut surface of the infected sweet potato. In transcriptomic analysis, a total of 13, 14, and 6 key genes in ipomeamarone, isochlorogenic acid, and l-tyrosine biosynthesis pathways, respectively, were identified. A conceptual model elucidating the physiological and molecular mechanism of -induced quality deterioration in sweet potato is proposed.
在储存过程中，诱导的甘薯品质劣变特征和机制尚不清楚。本研究调查了在贮藏过程中新乡甘薯根感染 后对其的影响。结果表明，感染后虽然没有外部症状，但当切割根时，感染根的切面迅速变黑，而未经处理的对照根则不受影响。通过高效液相色谱、代谢组学分析和 Illumina Novaseq 平台研究了感染和对照甘薯根之间的代谢物和转录差异。结果表明，在 28°C 下接种后 6 天储存时，组织中积累的有毒 ipomeamarone 高达 2.36 毫克/千克 DW，而对照组织样本中没有积累任何 ipomeamarone。代谢组学分析表明，感染组织中异绿原酸和 l-酪氨酸显著增加，与感染甘薯切面变黑有关。在转录组分析中，分别鉴定了 ipomeamarone、异绿原酸和 l-酪氨酸生物合成途径中的 13、14 和 6 个关键基因。提出了一个解释甘薯中 诱导的品质劣变的生理和分子机制的概念模型。
