Coolen R B, Herbstman R, Hermann P
Clin Chem. 1978 Sep;24(9):1636-8.
Creatine kinase isoenzyme I(BB) is generally not detectable in normal serum, and its occurrence in serum has been documented in only a few disease states. In particular, increased activity of this isoenzyme has been reported in association with chronic renal failure, hemodialysis, and renal transplantation. The present study demonstrates that the apparent creatine kinase observed in the serum of such renal patients is an artifact, observed as a result of measuring creatine kinase isoenzymes by fluorescence. Our observations resemble those of McKenzie et al. [Clin. Chim. Acta 70, 333(1976)] concerning an artifact in the fluorometric determination of lactate dehydrogenase isoenzymes in the sera of patients with end-stage renal failure. The artifact binds to albumin, is not a protein, and occurs in some normal sera at very low concentrations. This artifact can be mistakenly identified as isoenzyme I in renal-disease patients if CK isoenzymes are determined fluorometrically.
肌酸激酶同工酶I（BB）在正常血清中通常无法检测到，仅在少数疾病状态下血清中才会出现该同工酶。特别是，已有报道称该同工酶活性增加与慢性肾衰竭、血液透析及肾移植有关。本研究表明，在此类肾病患者血清中观察到的表观肌酸激酶是一种假象，这是通过荧光法测定肌酸激酶同工酶时观察到的结果。我们的观察结果与麦肯齐等人[《临床化学学报》70, 333(1976)]关于终末期肾衰竭患者血清中乳酸脱氢酶同工酶荧光测定中的一种假象的观察结果相似。该假象与白蛋白结合，不是一种蛋白质，且在一些正常血清中以非常低的浓度存在。如果通过荧光法测定CK同工酶，这种假象在肾病患者中可能会被误鉴定为同工酶I。
