Clin Lab. 2022 Jun 1;68(6). doi: 10.7754/Clin.Lab.2021.210626.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease that emerged in December 2019 from Wuhan, China, has led to a worldwide outbreak that has resulted in 234,809,103 confirmed cases and caused more than 4,800,375 deaths worldwide. MicroRNAs could be involved in the SARS-CoV-2 infection, but not many studies have been performed to explore this in postmortem cases. The purpose of our study is to evaluate the postmortem expression of microRNA-6501-5p, microRNA-5695, and microRNA-29b-3p from bronchial secretions in positive and negative SARS-CoV-2 deaths and to evaluate their usefulness as predictive biomarkers in the evolution of SARS-CoV-2 infection.
During the autopsy procedure on 61 "suspected" deaths at the Institute of Forensic Medicine in Timisoara, Romania, bronchial secretions were collected to detect SARS-CoV-2 infection postmortem. After the RT-PCR analysis, 44 SARS-CoV-2 cases were detected positive, while 17 cases were SARS-CoV-2 negative, which were considered as controls.
From the panel of microRNAs, microRNA-6501-5p, microRNA-5695, and microRNA-29b-3p were upregulated in SARS-CoV-2 cases and down-regulated in non-SARS-CoV-2 cases.
We concluded that using a panel of microRNAs as biomarkers in SARS-CoV-2 infection could aid in an early evaluation of the evolution of SARS-CoV-2-infected patients.
2019 年 12 月在中国武汉出现的严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）疾病导致了全球爆发，已确诊病例超过 2348.09103 万例，全球死亡人数超过 480.0375 万例。microRNA 可能参与 SARS-CoV-2 感染，但在死后病例中进行的研究并不多。我们的研究目的是评估支气管分泌物中 microRNA-6501-5p、microRNA-5695 和 microRNA-29b-3p 在 SARS-CoV-2 阳性和阴性死亡病例中的死后表达，并评估它们作为 SARS-CoV-2 感染演变的预测生物标志物的有用性。
在罗马尼亚蒂米什瓦拉法医研究所进行的 61 例“疑似”死亡尸检过程中，收集支气管分泌物以检测死后的 SARS-CoV-2 感染。RT-PCR 分析后，发现 44 例 SARS-CoV-2 病例呈阳性，17 例 SARS-CoV-2 病例呈阴性，作为对照。
在 microRNA 中，microRNA-6501-5p、microRNA-5695 和 microRNA-29b-3p 在 SARS-CoV-2 病例中上调，在非 SARS-CoV-2 病例中下调。
我们得出结论，使用 microRNA 作为 SARS-CoV-2 感染的生物标志物可以帮助早期评估 SARS-CoV-2 感染患者的病情演变。
