Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt.
Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt.
Microb Pathog. 2022 Aug;169:105613. doi: 10.1016/j.micpath.2022.105613. Epub 2022 Jun 12.
This study aimed to evaluate the immune response using the immune-regulating cytokines as (IL-1β; MHC-I and MHC-II) associated with co-infected sheep Oestrusovis and Coenurus cerebralis as well as oxidative stress markers (malondialdehyde "MDA" and nitric oxide "NO"). So; sheep samples from different regions in Egypt showed different neurological signs, were examined for detection of the cause of the nervous manifestations. Moreover, the O. ovis and C. cerebralis cysts were collected and identified using scanning electron microscopy. The brain tissues were evaluated for different immunological genes such as MHC-I, MHC-II, and Interleukin-1β activity using quantitative real-time PCR (qRT-PCR) techniques, where the infected sheep showed higher MHC-I gene expression (10-fold), higher MHC-II gene expression (peaked at 25-fold), and higher IL-1β gene expression (14-fold) than the control group. The MDA level was significantly increased. Also, stress marker (nitric oxide) levels were significantly higher in infectedsheep than in negative control one. During gross pathology, migrating larvae of O. ovis and C. cerebralis were noticed In such areas, hemorrhages and patches of clotted blood were noticed. cysts with prominent protoscolices were also observed and were attached to the caudal region near the cerebellum.
本研究旨在评估免疫反应，使用与绵羊 Oestrusovis 和 Coenurus cerebralis 混合感染相关的免疫调节细胞因子（IL-1β；MHC-I 和 MHC-II）以及氧化应激标志物（丙二醛“MDA”和一氧化氮“NO”）。因此；来自埃及不同地区的绵羊表现出不同的神经症状，对其进行检查以确定神经表现的原因。此外，使用扫描电子显微镜收集和鉴定 O.ovis 和 C. cerebralis 囊肿。使用定量实时 PCR（qRT-PCR）技术评估脑组织中的不同免疫基因，如 MHC-I、MHC-II 和白细胞介素-1β活性，其中感染绵羊的 MHC-I 基因表达（10 倍）、MHC-II 基因表达（峰值为 25 倍）和白细胞介素-1β基因表达（14 倍）均高于对照组。MDA 水平显著升高。此外，感染绵羊的应激标志物（一氧化氮）水平明显高于阴性对照组。在大体病理学检查中，注意到 O.ovis 和 C. cerebralis 的移行幼虫。在这些区域，注意到出血和血凝块斑块。还观察到带有明显原头蚴的囊肿，并附着在靠近小脑的尾侧区域。
