Clinical Pathology Department, Faculty of Medicine, Helwan University, Cairo, Egypt.
Community, Environmental and Occupational Medicine Department, Faculty of Medicine, Helwan University, Cairo, Egypt.
J Med Virol. 2022 Oct;94(10):4950-4958. doi: 10.1002/jmv.27935. Epub 2022 Jun 22.
Acute gastroenteritis is the main cause of mortality and morbidity in children worldwide. Studies stated that rotavirus and human adenovirus (HAdV) are common causes of nonbacterial gastroenteritis in children aged 0-5 years. The aim of this study was to determine the prevalence and the distribution of rotavirus, HAdV, and coinfections among hospitalized children with gastroenteritis below 7 years old and determine the prevalence of enteric HAdV among all HAdV gastroenteritis. The study was conducted on 150 children below 7 years old. Antigen detection for rotavirus and HAdV by ELISA and determination of enteric HAdV (serotype 40 and 41) by nested PCR and restriction endonucleases study were performed. Detection of rotavirus and HAdV antigens in 150 stool specimens from patients with gastroenteritis were 58% (87), 6.7% (10), and 8% (12) positive for rotavirus, HAdV, and coinfection, respectively. Out of 22 HAdV antigen-positive cases, 15 cases were positive by PCR for enteric HAdV, with the prevalence rate of enteric HAdV gastroenteritis among all HAdV gastroenteritis cases of 68%, a serotyping study by PCR detected serotype 40 in 46.7% of cases (7/15) and serotype 41 in 53.3% of cases (8/15) with no statistically significant difference between them. The study confirmed that rotavirus and HAdV are prevalent etiological agents of diarrhea in children below the school-age group, highlighting the necessity of the rotavirus vaccine in addition to the obligatory schedule of vaccines in Egypt. Also, it determined that the enteric HAdV gastroenteritis prevalence rate was 68% among all HAdV gastroenteritis.
急性胃肠炎是全球儿童死亡和发病的主要原因。研究表明，轮状病毒和人类腺病毒（HAdV）是 0-5 岁儿童非细菌性胃肠炎的常见病因。本研究旨在确定住院胃肠炎患儿中轮状病毒、HAdV 和混合感染的流行率和分布，并确定所有 HAdV 胃肠炎中肠型 HAdV 的流行率。该研究共纳入 150 名 7 岁以下儿童。采用 ELISA 检测轮状病毒和 HAdV 抗原，采用巢式 PCR 和限制性内切酶研究检测肠型 HAdV（血清型 40 和 41）。在 150 份胃肠炎患儿粪便标本中检测到轮状病毒和 HAdV 抗原的阳性率分别为 58%（87）、6.7%（10）和 8%（12）。在 22 例 HAdV 抗原阳性病例中，15 例通过 PCR 检测到肠型 HAdV 阳性，肠型 HAdV 胃肠炎在所有 HAdV 胃肠炎病例中的流行率为 68%，PCR 血清分型研究检测到 46.7%（7/15）的病例为血清型 40，53.3%（8/15）的病例为血清型 41，两者之间无统计学差异。该研究证实，轮状病毒和 HAdV 是学龄前儿童腹泻的常见病原体，这突出表明除了埃及强制性疫苗接种计划外，还需要接种轮状病毒疫苗。此外，该研究还确定肠型 HAdV 胃肠炎在所有 HAdV 胃肠炎中的流行率为 68%。
