Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan.
Faculty of Veterinary Medicine, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, the Netherlands.
Lett Appl Microbiol. 2022 Oct;75(4):908-912. doi: 10.1111/lam.13769. Epub 2022 Jun 24.
Clinical samples from 123 foals with suspected rhodococcosis submitted to the Veterinary Microbiological Diagnostic Centre of the Faculty of Veterinary Medicine between 1993 and 2006 were tested for the presence of the virulence gene vapA. Of the 123 samples, 120 were vapA-positive and 3 vapA-negative Rhodococcus equi were isolated. The 120 vapA-positive R. equi were isolated from 70 tracheal wash, 19 lung tissues, 7 lymph nodes, 6 synovial fluids, 13 abscesses or pus and single isolates from the uterus, gut, cerebrospinal fluid, abdomen fluid and faeces. Of the 120 isolates, 46 were from Dutch warmblood horses, 23 from Friesian horses, 14 from Trotters, 4 from Holsteiners, 3 from Arab breed, 2 from ponies, 1 from a Welsh pony and 27 from undefined breed horses. Using plasmid profile analysis of the 120 isolates, 117 isolates contained the 85-kb type I plasmid, 2 contained the 87-kb type I plasmid and 1 contained the novel 52-kb non-mobilizable virulence plasmid reported recently. These results showed that the virulent R. equi strains harbouring a virulence plasmid of 85-kb type I or 87-kb type I, which have been detected in clinical isolates from five European countries, are widespread in the Netherlands. This is the first report of plasmid types of clinical R. equi isolates in the Netherlands.
1993 年至 2006 年间，兽医微生物诊断中心收到了 123 例疑似马罗球菌病驹的临床样本，用于检测毒力基因 vapA 的存在。在 123 个样本中，120 个 vapA 阳性，3 个 vapA 阴性的马罗球菌被分离出来。120 个 vapA 阳性的马罗球菌分别从 70 个气管冲洗液、19 个肺组织、7 个淋巴结、6 个滑液、13 个脓肿或脓液以及子宫、肠道、脑脊液、腹部液体和粪便中分离出来。在 120 个分离株中，46 个来自荷兰温血马，23 个来自弗里西亚马，14 个来自特罗特马，4 个来自荷斯坦马，3 个来自阿拉伯马，2 个来自小马，1 个来自威尔士小马，27 个来自未定义品种的马。通过对 120 个分离株的质粒图谱分析，117 个分离株含有 85-kb 型 I 质粒，2 个分离株含有 87-kb 型 I 质粒，1 个分离株含有最近报道的新型 52-kb 非可移动毒力质粒。这些结果表明，携带 85-kb 型 I 或 87-kb 型 I 毒力质粒的毒力马罗球菌菌株在五个欧洲国家的临床分离株中广泛存在，在荷兰也很普遍。这是荷兰首次报道临床马罗球菌分离株的质粒类型。
