• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

肿瘤抑制因子 arhgap28 和视网膜母细胞瘤在蜥蜴壁蜥中的免疫定位表明它们有助于尾巴的有调节再生。

Immunolocalization of tumor suppressors arhgap28 and retinoblastoma in the lizard Podarcis muralis suggests that they contribute to the regulated regeneration of the tail.

机构信息

Comparative Histolab, Padova, Italy.

Department of Biology, University of Bologna, Bologna, Italy.

出版信息

J Morphol. 2022 Jul;283(7):973-986. doi: 10.1002/jmor.21484. Epub 2022 Jun 16.

DOI:10.1002/jmor.21484
PMID:35708299
Abstract

Tail regeneration in lizards is an outstanding and unique postembryonic morphogenetic process. This developmental process is regulated by poorly known factors, but recent studies have suggested that it derives from a balanced activity between oncoproteins and tumor suppressors. Transcriptome and expression data have indicated that arhgap28 and retinoblastoma proteins are among the main tumor suppressors activated during tail regeneration. However, their cellular localization is not known. Therefore, in the present immunohistochemical study, two proteins have been detected in various tissues at the beginning of their differentiation. Both proteins are present especially in the new scales, axial cartilage, and muscle bundles of the regenerating tail, the main tissues forming the new tail. Sparse or occasionally labeled cells are observed in the blastema, but intense labeling is seen in the basal layers of the wound (regenerating) epidermis and in external differentiating epidermal layers. Numerous keratinocytes also show a nuclear localization for both proteins, suggesting that the latter may activate a gene program for tissue differentiation after the inhibition of cell multiplication. Based on microscopic, molecular, experimental, and in vitro studies, a hypothesis on the "inhibition of contact" among the apical cells of the blastema and those of proximal differentiating tissues is proposed to explain the permanence of an active blastema only at the apex of the regenerating tail without tail growth can degenerate into a tumorigenic outgrowth.

摘要

蜥蜴的尾部再生是一个出色而独特的胚胎后形态发生过程。这个发育过程受未知因素的调控,但最近的研究表明,它源于癌蛋白和肿瘤抑制因子之间的平衡活性。转录组和表达数据表明, arhgap28 和视网膜母细胞瘤蛋白是在尾部再生过程中激活的主要肿瘤抑制因子之一。然而,它们的细胞定位尚不清楚。因此,在本免疫组织化学研究中,在分化开始时检测到两种蛋白在各种组织中的存在。这两种蛋白在再生尾巴的新鳞片、轴性软骨和肌肉束中特别存在,这些是形成新尾巴的主要组织。在芽基中观察到稀疏或偶尔标记的细胞,但在伤口(再生)表皮的基底层和外部分化的表皮层中观察到强烈的标记。许多角质形成细胞也显示出这两种蛋白的核定位,表明后者可能在细胞增殖被抑制后激活组织分化的基因程序。基于显微镜、分子、实验和体外研究,提出了一个关于芽基的顶端细胞和近端分化组织之间“接触抑制”的假说,以解释为什么只有在再生尾巴的顶端才会保持活跃的芽基,而没有尾巴生长的芽基会退化并形成肿瘤性赘生物。

相似文献

1
Immunolocalization of tumor suppressors arhgap28 and retinoblastoma in the lizard Podarcis muralis suggests that they contribute to the regulated regeneration of the tail.肿瘤抑制因子 arhgap28 和视网膜母细胞瘤在蜥蜴壁蜥中的免疫定位表明它们有助于尾巴的有调节再生。
J Morphol. 2022 Jul;283(7):973-986. doi: 10.1002/jmor.21484. Epub 2022 Jun 16.
2
Immunodetection of ephrin receptors in the regenerating tail of the lizard suggests stimulation of differentiation and muscle segmentation.免疫检测蜥蜴再生尾部的 Ephrin 受体提示其能刺激分化和肌肉分段。
Zool Res. 2019 Sep 18;40(5):416-426. doi: 10.24272/j.issn.2095-8137.2019.046.
3
Review: Regeneration of the tail in lizards appears regulated by a balanced expression of oncogenes and tumor suppressors.综述:蜥蜴的尾巴再生似乎受到癌基因和肿瘤抑制基因的平衡表达调控。
Ann Anat. 2022 Jan;239:151824. doi: 10.1016/j.aanat.2021.151824. Epub 2021 Aug 31.
4
Immunolocalization of adenomatous polyposis coli protein (apc) in the regenerating lizard tail suggests involvement in tissue differentiation and regulation of growth.腺瘤性结肠息肉蛋白(APC)在再生蜥蜴尾部的免疫定位表明其参与组织分化和生长调控。
J Morphol. 2022 May;283(5):677-688. doi: 10.1002/jmor.21465. Epub 2022 Mar 2.
5
Immunolocalization of Wnts in the lizard blastema supports a key role of these signaling proteins for tail regeneration.Wnt蛋白在蜥蜴再生芽基中的免疫定位支持了这些信号蛋白在尾巴再生中发挥关键作用。
J Morphol. 2020 Jan;281(1):68-80. doi: 10.1002/jmor.21080. Epub 2019 Nov 13.
6
Autoradiography and inmmunolabeling suggests that lizard blastema contains arginase-positive M2-like macrophages that may support tail regeneration.放射自显影和免疫标记表明,蜥蜴的芽基含有精氨酸酶阳性的 M2 样巨噬细胞,这些细胞可能支持尾巴再生。
Ann Anat. 2020 Sep;231:151549. doi: 10.1016/j.aanat.2020.151549. Epub 2020 Jun 5.
7
Immunolocalization of Nestin in the lizard Podarcis muralis indicates up-regulation during the process of tail regeneration and epidermal differentiation.在蜥蜴壁蜥的尾巴再生和表皮分化过程中巢蛋白的免疫定位表明其表达上调。
Ann Anat. 2014 May;196(2-3):135-43. doi: 10.1016/j.aanat.2013.12.004. Epub 2013 Dec 25.
8
Immunolocalization of FGF7 (KGF) in the regenerating tail of lizard suggests it is involved in the differentiation of the epidermis.成纤维细胞生长因子7(KGF)在蜥蜴再生尾巴中的免疫定位表明,它参与了表皮的分化。
Acta Histochem. 2015 Oct;117(8):718-24. doi: 10.1016/j.acthis.2015.09.003. Epub 2015 Oct 25.
9
Immunohistochemical and western blot analysis suggest that the soluble forms of FGF1-2 and FGFR1-2 sustain tail regeneration in the lizard.免疫组织化学和蛋白质印迹分析表明,FGF1 - 2和FGFR1 - 2的可溶性形式维持蜥蜴的尾巴再生。
Ann Anat. 2017 Nov;214:67-74. doi: 10.1016/j.aanat.2017.07.005. Epub 2017 Aug 18.
10
Immunolocalization of FGF8/10 in the Apical Epidermal Peg and Blastema of the regenerating tail in lizard marks this apical growing area.成纤维细胞生长因子8/10在蜥蜴再生尾巴的顶端表皮钉和芽基中的免疫定位标记了这个顶端生长区域。
Ann Anat. 2016 Jul;206:14-20. doi: 10.1016/j.aanat.2016.03.010. Epub 2016 Apr 23.

引用本文的文献

1
Epidermal Differentiation Genes of the Common Wall Lizard Encode Proteins with Extremely Biased Amino Acid Contents.常见壁蜥的表皮分化基因编码具有极度偏倚性氨基酸组成的蛋白质。
Genes (Basel). 2024 Aug 28;15(9):1136. doi: 10.3390/genes15091136.