基于基于发夹组装介导的 CRISPR-Cas12a 系统的卵壳金@硫化镉上 microRNA 的光电化学生物分析。
Photoelectrochemical bioanalysis of microRNA on yolk-in-shell Au@CdS based on the catalytic hairpin assembly-mediated CRISPR-Cas12a system.
机构信息
Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, P. R. China.
出版信息
Chem Commun (Camb). 2022 Jul 5;58(54):7562-7565. doi: 10.1039/d2cc02821b.
This work reports on the proof-of-concept of a photoelectrochemical (PEC) biosensor with a horseradish peroxidase-single stranded DNA-encoded magnetic bead (MB-ssDNA-HRP) signal probe cleaved by the catalytic hairpin assembly (CHA)-mediated clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a system for the quantification of microRNA (miR-21) by using yolk-in-shell Au@CdS as a photoactive material.
本工作报道了一种基于光电流（PEC）的生物传感器的概念验证，该传感器使用辣根过氧化物酶-单链 DNA 编码的磁性珠（MB-ssDNA-HRP）信号探针，该探针可被催化发夹组装（CHA）介导的簇状规则间隔短回文重复序列（CRISPR）-Cas12a 系统切割，用于通过蛋黄壳型 Au@CdS 作为光活性材料定量检测 microRNA（miR-21）。
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