Liaoning Key Laboratory of Lignocellulose Chemistry and Biomaterials, Dalian Polytechnic University, Dalian, Liaoning 116034, China.
Department of Cardiology, Xi'an No. 3 Hospital, the Affiliated Hospital of Northwest University, Xi'an 710018, China.
Int J Biol Macromol. 2022 Aug 1;214:170-180. doi: 10.1016/j.ijbiomac.2022.06.066. Epub 2022 Jun 14.
The construction of lignin nanoparticles (LNPs) with both lignin properties and nanomaterial properties through controlling the morphologies and structures of lignin is one of the effective ways to realize its application in the field of biomedicine. Firstly, the morphology and chemical structure of LNPs were studied in detailed. The results showed that the chemical structural characteristics of LNPs had not changed significantly and its morphology was more regular shape and narrower size distribution (50-350 nm). Besides, LNPs also exhibited excellent water dispersion stability and high negative zeta potential. Subsequently, LNPs as wound dressings had good antioxidant property, excellent adsorption capacity of protein, outstanding bactericidal activity and remarkable biocompatibility, suggesting that LNPs did not interfere with cell proliferation during wound healing. Finally, the in vivo results of mouse wounds further illustrated that treatment of wounded skin wounds with LNPs enhanced its effective healing. After 15 days, as compared with the untreated control and original lignin (OL) groups, the wounds treated of LNPs was completely closed and granulation tissue formation was advanced. Overall, this study can be a good method for high-value applications of LNPs, and highlighting the advantages of using lignin as medical adjuvant nanomaterials to accelerate wound healing.
通过控制木质素的形态和结构，构建兼具木质素性质和纳米材料性质的木质素纳米颗粒（LNP），是实现其在生物医药领域应用的有效途径之一。首先，详细研究了 LNP 的形态和化学结构。结果表明，LNP 的化学结构特征没有发生明显变化，其形态更规则，粒径分布更窄（50-350nm）。此外，LNP 还表现出优异的水分散稳定性和高负 zeta 电位。随后，作为伤口敷料的 LNP 具有良好的抗氧化性能、优异的蛋白质吸附能力、出色的杀菌活性和显著的生物相容性，表明 LNP 在伤口愈合过程中不会干扰细胞增殖。最后，小鼠伤口的体内结果进一步表明，用 LNP 处理受伤皮肤伤口可增强其有效愈合。与未处理的对照组和原始木质素（OL）组相比，在 15 天后，LNP 处理的伤口完全闭合，肉芽组织形成得到了促进。总体而言，这项研究可为 LNP 的高价值应用提供一种很好的方法，并突出了使用木质素作为医疗辅助纳米材料来加速伤口愈合的优势。
