Cao Zhenping, Wang Lu, Liu Rui, Lin Sisi, Wu Feng, Liu Jinyao
Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Institute of Molecular Medicine, State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Mater Today Bio. 2022 Jun 6;15:100311. doi: 10.1016/j.mtbio.2022.100311. eCollection 2022 Jun.
The mammalian microbiota plays essential roles in health. A primary determinant to understand the interaction with the host is the distribution and viability of its key microorganisms. Here, a strategy of encoding with a fluorescence-activating and absorption-shifting tag (FAST) is reported to prepare living bacterial probes for real-time dynamic, dual-modal, and molecular oxygen-independent imaging of the host microbiota. Carrying FAST endows bacteria with rapid on-demand turn on-off fluorescence by adding or removal of corresponding fluorogens. Encoded bacteria are able to reversibly switch emission bands for dual-color fluorescence imaging via fluorogen exchange. Due to molecular oxygen-independent emission of FAST, encoded bacteria can emit fluorescence under anaerobic environments including the gut and tumor. These living probes demonstrate the applicability to quantify the vitality of bacteria transplanted to the gut microbiota. This work proposes a unique fluorescence probe for investigating the dynamics of the host microbiota.
哺乳动物微生物群对健康起着至关重要的作用。了解其与宿主相互作用的一个主要决定因素是其关键微生物的分布和活力。在此，报道了一种用荧光激活和吸收转移标签（FAST）进行编码的策略，用于制备活细菌探针，以对宿主微生物群进行实时动态、双模态和不依赖分子氧的成像。携带FAST通过添加或去除相应的荧光原赋予细菌快速按需开启或关闭荧光的能力。编码细菌能够通过荧光原交换可逆地切换发射带以进行双色荧光成像。由于FAST不依赖分子氧发射，编码细菌可以在包括肠道和肿瘤在内的厌氧环境中发出荧光。这些活探针证明了其在量化移植到肠道微生物群中的细菌活力方面的适用性。这项工作提出了一种独特的荧光探针，用于研究宿主微生物群的动态变化。
