Nakashima K, Umekawa C, Yoshida H, Nakatsuji S, Akiyama S
J Chromatogr. 1987 Feb 20;414(1):11-7. doi: 10.1016/0378-4347(87)80019-1.
The selective determination of thiols in biological samples was investigated by high-performance liquid chromatography using N-[4-(6-dimethylamino-2-benzofuranyl)phenyl] maleimide, which was found to give fluorescent products when treated with certain thiols. Six kinds of thiol (reduced glutathione, cysteine, N-acetylcysteine, cysteamine, homocysteine and coenzyme A) could be separated simultaneously within ca. 12 min and determined at final level of sensitivity. The method was successfully applied to the determination of thiols in rat tissues and plasma and in human normal serum.
使用N-[4-(6-二甲基氨基-2-苯并呋喃基)苯基]马来酰亚胺，通过高效液相色谱法对生物样品中的硫醇进行选择性测定。结果发现，该物质与某些硫醇反应时会生成荧光产物。六种硫醇（还原型谷胱甘肽、半胱氨酸、N-乙酰半胱氨酸、半胱胺、同型半胱氨酸和辅酶A）能够在约12分钟内同时分离，并在最终灵敏度水平下进行测定。该方法成功应用于大鼠组织、血浆以及人正常血清中硫醇的测定。
