Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
Key Laboratory of Sustainable Forest Ecosystem Management-Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
Pestic Biochem Physiol. 2022 Jun;184:105079. doi: 10.1016/j.pestbp.2022.105079. Epub 2022 Mar 14.
Variety of diuretic hormone neuropeptides is known to regulate water and ion balance in invertebrates. By activating their specific neuropeptide, diuretic hormone receptor (DHR) transmits extracellular signals into the cell, and then produces functional cell activity, which plays an important role in regulating physiology and behavior. However, little is known about the function of DHR gene in Lymantria dispar. DHR gene was firstly identified in L. dispar and its physiological functions were investigated using RNA interference (RNAi) technology. The results showed that except for the 6 instar larvae, the expression levels of DHR gene in the larval stages are higher than that in the egg, pupal and adult stages. The DHR gene is highly expressed in hindgut and midgut tissues. The L. dispar larvae significantly increased their water content and high temperature tolerance after the DHR was silenced, while decreasing excretion and feeding behavior. The physiological function of DHR is associated with desiccation, high temperature and starvation resistance. DHR could contribute to future development of novel insecticide to manage this global forest pest population.
已知各种利尿激素神经肽可调节无脊椎动物的水和离子平衡。利尿激素受体（DHR）通过激活其特定的神经肽，将细胞外信号传入细胞，然后产生功能性的细胞活性，在调节生理和行为方面发挥着重要作用。然而，关于 DHR 基因在舞毒蛾中的功能知之甚少。首次在舞毒蛾中鉴定出 DHR 基因，并利用 RNA 干扰（RNAi）技术研究了其生理功能。结果表明，除了 6 龄幼虫外，DHR 基因在幼虫期的表达水平均高于卵、蛹和成虫期。DHR 基因在中肠和后肠组织中高度表达。沉默 DHR 后，舞毒蛾幼虫的水分含量显著增加，高温耐受性提高，而排泄和摄食行为减少。DHR 的生理功能与抗干燥、高温和饥饿有关。DHR 可能有助于未来开发新型杀虫剂来管理这种全球性的森林害虫种群。
