探索甲基乙二醛与 NH 或丙氨酸的聚合反应，以分析类黑精中典型聚合物的形成。
Exploring polymerisation of methylglyoxal with NH or alanine to analyse the formation of typical polymers in melanoidins.
机构信息
State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China; School of Public Health, Zunyi Medical University, Zunyi 563000, China.
State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
出版信息
Food Chem. 2022 Nov 15;394:133472. doi: 10.1016/j.foodchem.2022.133472. Epub 2022 Jun 11.
To investigate the formation of typical melanoidin polymers, methylglyoxal (MGO) with NH or alanine (Ala) was used to form coloured compounds, with glyoxal or acetone used as controls. The products were characterised using chromatography, mass spectrometry, and spectroscopy. Spectroscopic results showed that the coloured compounds formed were similar to melanoidins in food. GC-MS results showed that the MGO-based reaction generated similar volatile compounds using the Maillard reaction. Mass spectrometry showed that the molecular weights of structural units in the polymers were mainly 162, 169, and 176 Da, and these could be reassembled using the basic units derived from MGO alone or in combination with nitrogen. Hence, polymers recombined using basic structural units should be considered while determining melanoidin biomarkers. The preparation of coloured compounds using MGO with NH can be used as a novel method to produce the control compounds for melanoidin after process optimization.
为了研究典型类黑素聚合物的形成，使用甲基乙二醛（MGO）与 NH 或丙氨酸（Ala）形成有色化合物，以乙醛或丙酮作为对照。使用色谱、质谱和光谱法对产物进行了表征。光谱结果表明，形成的有色化合物与食品中的类黑素相似。GC-MS 结果表明，MGO 基反应产生了与美拉德反应类似的挥发性化合物。质谱表明，聚合物中结构单元的分子量主要为 162、169 和 176 Da，可以使用单独或与氮结合的 MGO 衍生的基本单元重新组装。因此，在确定类黑素生物标志物时，应考虑使用基本结构单元重新组合的聚合物。使用 MGO 与 NH 制备有色化合物可以作为一种新方法，在优化工艺后用于生产类黑素的对照化合物。
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