Testicular acid phosphatases of the mouse. Characterization after chromatographic fractionation.

The testicular homogenate of adult mice was eluted by DEAE-cellulose chromatography. Four peaks of acid phosphatase were identified. The pooled samples of each peak were used to determine substrate specificity, pH-optima, modifier characteristics, thermal stability, and Km-values. The results obtained suggest that each peak may represent a separate and distinct type of acid phosphatase. These observations are very similar to those previously obtained with the rat testis. The acid phosphatase activities in the mouse seem, however, to differ biochemically to some extent from those in the rat.