Wu Rina, Xu Bo, Shi Fengling
Key Laboratory of Grassland Resources of the Ministry of Education, College of Grassland Resources and Environment, Inner Mongolia Agricultural University, Hohhot, China.
Front Plant Sci. 2022 Jun 2;13:907674. doi: 10.3389/fpls.2022.907674. eCollection 2022.
Abiotic stresses affect plant growth and productivity. The outstanding stress resistance of makes it a desirable gene resource to improve the stress tolerance of other plants. The roles of three differently expressed genes [(DEGs) (, , and )] from in stress resistance have not been fully elucidated. Therefore, we constructed their expression vectors, transformed them into tobacco, and subjected transgenic lines to abiotic stresses. Through comprehensive bioinformatics, transcriptomic, morphological, and physiological analyses of transgenic lines, we have revealed the critical role of these three DEGs in plant growth and abiotic stress response. The upregulation of genes enhanced the germination rate, biomass, root length number, etc. Additionally, the accumulation of osmolytes increased the activity of antioxidant enzymes. These genes are also associated with improved seed yield, increased branching, and early flowering, thereby shortening the growth period. Potentially, this is one of the ways for tobacco to cope with stress. Furthermore, the resistance of transgenic tobacco expressing or was better than that with . and can improve drought and salt tolerance of plants, whereas is beneficial in improving drought and cold resistance. Moreover, or can promote root elongation and increase the root number, whereas mainly promotes root elongation. This may be the reason why stress resistance conferred by is weaker than that associated with the other two genes. Overall, , , and positively modulate plant growth and stress tolerance.
非生物胁迫影响植物生长和生产力。[具体植物名称]的卓越抗逆性使其成为改善其他植物胁迫耐受性的理想基因资源。来自[具体植物名称]的三个差异表达基因（DEGs）（[基因1名称]、[基因2名称]和[基因3名称]）在抗逆性中的作用尚未完全阐明。因此，我们构建了它们的表达载体，将其转化到烟草中，并对转基因株系施加非生物胁迫。通过对转基因株系进行全面的生物信息学、转录组学、形态学和生理学分析，我们揭示了这三个DEGs在植物生长和非生物胁迫响应中的关键作用。[基因1名称]基因的上调提高了发芽率、生物量、根长数量等。此外，渗透调节物质的积累增加了抗氧化酶的活性。这些基因还与种子产量提高、分枝增加和早花有关，从而缩短了生长周期。这可能是烟草应对胁迫的方式之一。此外，表达[基因2名称]或[基因3名称]的转基因烟草的抗性优于表达[基因1名称]的。[基因2名称]和[基因3名称]可以提高植物的耐旱性和耐盐性，而[基因1名称]有利于提高抗旱性和抗寒性。此外，[基因2名称]或[基因3名称]可以促进根伸长并增加根数量，而[基因1名称]主要促进根伸长。这可能是[基因1名称]赋予的抗逆性弱于其他两个基因的原因。总体而言，[基因1名称]、[基因2名称]和[基因3名称]正向调节植物生长和胁迫耐受性。
