Bentley M D, Vanhoutte V, Schryver S M, Romero J C, Vanhoutte P M
J Lab Clin Med. 1987 May;109(5):595-600.
The contractile effects of a commonly used radiographic contrast medium, sodium diatrizoate and meglumine diatrizoate, were examined on isolated canine renal arteries. Rings of renal artery, with and without endothelium, were mounted in organ chambers filled with Krebs-Ringer bicarbonate solution to record isometric tension. Both preparations contracted comparably to increasing concentrations (10(-9) to 10(-4) mol/L) of norepinephrine, and only rings with endothelium relaxed with the application of acetylcholine. The contractile responses of the rings to increasing concentrations of either contrast medium or osmotically matched solutions of glucose were similar, suggesting that the primary factor for contraction was the osmolality of the bathing solution. No significant differences were observed between the responses of rings with and without endothelium, suggesting that the endothelium did not influence the contractile response. After exposure to the hyperosmotic solutions, rings were again tested for relaxation with acetylcholine. Rings that were prepared with endothelium relaxed with the application of acetylcholine, suggesting that functional properties of the endothelium were not affected by the hyperosmotic media. The results of this study indicate that the vasoconstrictor effects of radiographic contrast media such as meglumine/sodium diatrizoate are related to increased osmolality and are not influenced by the presence of endothelium.
研究了常用的造影剂泛影葡胺和泛影钠对犬离体肾动脉的收缩作用。将有内皮和无内皮的肾动脉环安装在充满 Krebs-Ringer 碳酸氢盐溶液的器官浴槽中，记录等长张力。两种制剂对去甲肾上腺素浓度增加（10(-9)至 10(-4)mol/L）的收缩反应相当，且只有有内皮的血管环在应用乙酰胆碱时会舒张。血管环对造影剂或等渗葡萄糖溶液浓度增加的收缩反应相似，表明收缩的主要因素是浴液的渗透压。有内皮和无内皮的血管环反应之间未观察到显著差异，表明内皮不影响收缩反应。在暴露于高渗溶液后，再次用乙酰胆碱测试血管环的舒张情况。有内皮的血管环在应用乙酰胆碱时舒张，表明内皮的功能特性不受高渗介质的影响。本研究结果表明，泛影葡胺/泛影钠等造影剂的血管收缩作用与渗透压升高有关，且不受内皮存在的影响。
