Andersen B, Osborn M, Weber K
Cytobiologie. 1978 Aug;17(2):354-64.
Monospecific antibodies against the homogeneous Ca++ dependent regulatory protein of cyclic nucleotide phosphodiesterase (CDR protein) from bovine brain were used in indirect immunofluorescence microscopy to visualize the cytoplasmic organization of this key regulatory protein in growing tissue culture cells. Although cells during interphase reveal only a weak general cytoplasmic fluorescence, a dramatic reorganization of CDR protein occurs with the onset of mitosis. Throughout the different mitotic stages CDR protein is strongly concentrated in the two polar parts of each half spindle. After completion of telophase (CDR protein appears at both cytoplasmic ends of the intercellular bridge which still connects the two daughter cells. Parallel use of monospecific antibodies against CDR protein and tubulin emphasizes the spatial restriction in the localization of CDR protein during mitosis and early G1 phase of the cell cycle.
针对来自牛脑的环状核苷酸磷酸二酯酶的均一钙依赖性调节蛋白（CDR蛋白）的单特异性抗体，被用于间接免疫荧光显微镜观察，以可视化生长中的组织培养细胞中这种关键调节蛋白的细胞质组织。虽然间期细胞仅显示出微弱的总体细胞质荧光，但随着有丝分裂的开始，CDR蛋白会发生显著的重新组织。在整个不同的有丝分裂阶段，CDR蛋白强烈集中在每个半纺锤体的两个极区。末期完成后（CDR蛋白出现在仍连接两个子细胞的细胞间桥的两个细胞质末端。针对CDR蛋白和微管蛋白的单特异性抗体的平行使用强调了在有丝分裂和细胞周期的早期G1期CDR蛋白定位的空间限制。
