小鼠补体的第五成分（C5）。缺陷分子基础分析。
The fifth component of complement (C5) in the mouse. Analysis of the molecular basis for deficiency.
作者信息
Wheat W H, Wetsel R, Falus A, Tack B F, Strunk R C
出版信息
J Exp Med. 1987 May 1;165(5):1442-7. doi: 10.1084/jem.165.5.1442.
Abstract
C5-deficient mice differed from C5-sufficient mice both quantitatively and qualitatively in C5 protein, C5 mRNA, and the C5 gene. C5-deficient protein was present as decreased amounts of an unprocessed, single-chain precursor. C5-deficient mRNA was decreased in amount and present in two forms, the smaller of which was the same as the single form in normal cells. Nuclei from both normal and deficient cells contained the larger form of C5 mRNA, and C5-deficient DNA demonstrated differences from the normal pattern on Southern analysis for two restriction enzymes. These data suggest that the primary transcript of the C5-deficient gene is abnormal, retarding the processing of the C5 mRNA, and that the C5-deficient mRNA codes for an abnormal protein.
摘要
C5缺陷小鼠在C5蛋白、C5信使核糖核酸（mRNA）和C5基因方面，在数量和质量上均与C5充足的小鼠有所不同。C5缺陷蛋白以未加工的单链前体数量减少的形式存在。C5缺陷mRNA的数量减少，并以两种形式存在，其中较小的一种与正常细胞中的单一形式相同。正常细胞和缺陷细胞的细胞核均含有较大形式的C5 mRNA，并且在针对两种限制性内切酶的Southern分析中，C5缺陷DNA显示出与正常模式的差异。这些数据表明，C5缺陷基因的初级转录本是异常的，阻碍了C5 mRNA的加工，并且C5缺陷mRNA编码一种异常蛋白。
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