Beijing Key laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing, China.
Luminescence. 2022 Sep;37(9):1427-1435. doi: 10.1002/bio.4314. Epub 2022 Jun 28.
A novel D-π-A type fluorescent probe (probe 1) was developed for water content detection in organic solvents. By analyzing the relationship between fluorescence and water content, the probe was successfully applied to determine trace water content in tetrahydrofuran, ethyl acetate, 2-butanone, acetone, dimethylformamide, and acetonitrile. High water content in THF and ethyl acetate was associated with a gradual colour change from yellowish green to earthy yellow. The red/green value had a linear relationship with the water content in THF and ethyl acetate. There was a linear relationship between the red/blue value and water content in 2-butanone and acetone. Furthermore, probe 1 could be used for human serum albumin detection. Unexpectedly, probe 1 had a different colour response in deuterated and nondeuterated solvents, and had different fluorescence intensity and fluorescence emission wavelength. Probe 1 is rare tool that can distinguish between deuterated and nondeuterated reagents.
一种新型的 D-π-A 型荧光探针（探针 1）被开发用于有机溶剂中水含量的检测。通过分析荧光与水含量的关系，成功地将探针应用于四氢呋喃、乙酸乙酯、2-丁酮、丙酮、二甲基甲酰胺和乙腈中痕量水含量的测定。THF 和乙酸乙酯中的高含水量导致颜色从黄绿色逐渐变为土黄色。红/绿值与 THF 和乙酸乙酯中的含水量呈线性关系。在 2-丁酮和丙酮中，红/蓝值与水含量呈线性关系。此外，探针 1 可用于人血清白蛋白的检测。出乎意料的是，探针 1 在氘代和非氘代溶剂中具有不同的颜色响应，并且具有不同的荧光强度和荧光发射波长。探针 1 是一种罕见的工具，可以区分氘代和非氘代试剂。
