揭示多聚六亚甲基胍在污泥发酵过程中对抗生素抗性基因传播的风险和关键机制。
Unveiling the risks and critical mechanisms of polyhexamethylene guanidine on the antibiotic resistance genes propagation during sludge fermentation process.
机构信息
Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, China.
出版信息
Bioresour Technol. 2022 Sep;359:127488. doi: 10.1016/j.biortech.2022.127488. Epub 2022 Jun 17.
This study mainly investigated the environmental risks of polyhexamethylene guanidine (PHMG) occurred in waste activated sludge (WAS) on the antibiotic resistance genes (ARGs) spread during anaerobic fermentation, and disclosed the critical mechanisms. The total ARGs abundance was increased by 32.2-46.4% at different stressing levels of PHMG. The main resistance mechanism categories of ARGs shifted to the target alternation and efflux pump. PHMG disintegrated WAS structure and increased the cell permeability, which benefitted the mobile genetic elements (MGEs) release and horizontal transfer of ARGs. Besides, PHMG induced the enrichment of potential ARGs hosts (i.e., Burkholderia, Bradyrhizobium and Aeromonas). Moreover, PHMG upregulated the metabolic pathways (i.e., two-component system, quorum sensing, and ATP-binding cassette transporters) and critical genes expression (i.e., metN, metQ, rpfF, rstA and rstB) related with ARGs generation and dissemination. Structural equation model analysis revealed that microbial community structure was the predominant contributor to the ARGs propagation.
本研究主要考察了废活性污泥（WAS）中聚六亚甲基胍（PHMG）引发的环境风险对厌氧发酵过程中抗生素抗性基因（ARGs）传播的影响，并揭示了关键机制。在不同 PHMG 胁迫水平下，总 ARGs 丰度增加了 32.2-46.4%。ARGs 的主要抗性机制类别向靶位改变和外排泵转移。PHMG 破坏了 WAS 的结构并增加了细胞通透性，有利于移动遗传元件（MGEs）的释放和 ARGs 的水平转移。此外，PHMG 诱导了潜在 ARGs 宿主（如伯克霍尔德氏菌、慢生根瘤菌和气单胞菌）的富集。此外，PHMG 上调了与 ARGs 产生和传播相关的代谢途径（如双组分系统、群体感应和 ATP 结合盒转运蛋白）和关键基因表达（如 metN、metQ、rpfF、rstA 和 rstB）。结构方程模型分析表明，微生物群落结构是 ARGs 传播的主要贡献者。
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