National Research Council of Italy, Institute of Biomolecular Chemistry, Pozzuoli, Naples, Italy.
Methods Mol Biol. 2022;2498:307-313. doi: 10.1007/978-1-0716-2313-8_16.
Marine organisms are well known for their capability to produce chemically unique and biological relevant small molecules. However, marine extracts are complex mixtures of compounds with a huge presence of salts. Thus, biological screening and chemical analysis of marine extracts pose specific technical constraints and require adequate sample preparation. We have developed an automated solid-phase extraction (SPE)-based method to desalt marine extracts and recover metabolites, adapt to be integrated in platform of high-throughput screening. The procedure uses a poly(styrene-divynylbenzene)-based support and a stepwise organic solvent elution herein described.
海洋生物以其产生具有化学独特性和生物相关性的小分子的能力而闻名。然而，海洋提取物是化合物的复杂混合物，其中含有大量盐分。因此，海洋提取物的生物筛选和化学分析存在特定的技术限制，需要进行适当的样品制备。我们开发了一种基于自动化固相萃取 (SPE) 的方法来脱盐海洋提取物并回收代谢物，使其适应高通量筛选平台的集成。该方法使用基于聚苯乙烯-二乙烯基苯的载体，并采用本文所述的分步有机溶剂洗脱。
