Bu Yanling, Li Qiang, Liang Xiao, Gao Qi, Ma Xiaoming, Jin Minghui, Yang Xiuling
Department of Ultrasonography, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, China.
Department of Cardiology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, China.
Gynecol Obstet Invest. 2022;87(3-4):211-218. doi: 10.1159/000525594. Epub 2022 Jun 21.
Ultrasound-targeted microbubble destruction (UTMD) is an effective technology for microRNA (miRNA) delivery. miR-1228 plays a crucial role and acts as an oncogenic role in several types of cancers. This study aimed to investigate the functional effect of UTMD-mediated miR-1228 knockdown in cervical-cancer cells.
A total of 131 patients who were diagnosed with cervical cancer by histopathological examination were enrolled in this study at the Third Affiliated Hospital of Qiqihar Medical University from February 2018 to January 2021.
PARTICIPANTS/MATERIALS, SETTING, METHODS: miR-1228 expression was tested by reverse-transcription quantitative PCR assay. miR-1228 inhibitors were transfected into cervical-cancer cells using the UTMD method. Then, Cell Counting Kit-8 and transwell assays were carried out to explore the cell proliferation, migration, and invasion potentials, respectively.
The results revealed that miR-1228 expression was high in human cervical-cancer tissues and cell lines. Knockdown of miR-1228 suppressed tumor cell proliferation abilities, migration, and invasion capacities. Moreover, UTMD-mediated mIR-1228 inhibitor delivery enhanced the transfection efficiency of miR-1228 inhibitor alone. The UTMD-mediated miR-1228 inhibition enhanced the suppressive role of miR-1228 downregulation on cell proliferation capacity, migration, and invasion abilities in tumor cells, compared to miR-1228 knockdown alone.
The experiments were carried out only in SiHa and HeLa cells in vitro, and the results were not verified in animals.
These results indicated that the delivery of the UTMD-mediated miR-1228 inhibitor might be a potential therapeutic method for the treatment of cervical cancer through suppressing cellular activities.
超声靶向微泡破坏（UTMD）是一种有效的微小RNA（miRNA）递送技术。miR-1228在多种癌症中发挥关键作用并具有致癌作用。本研究旨在探讨UTMD介导的miR-1228敲低对宫颈癌细胞的功能影响。
2018年2月至2021年1月，齐齐哈尔医学院附属第三医院共纳入131例经组织病理学检查确诊为宫颈癌的患者。
参与者/材料、设置、方法：采用逆转录定量PCR法检测miR-1228表达。使用UTMD方法将miR-1228抑制剂转染至宫颈癌细胞中。然后，分别进行细胞计数试剂盒-8和Transwell实验，以探讨细胞增殖、迁移和侵袭潜能。
结果显示，miR-1228在人宫颈癌组织和细胞系中高表达。敲低miR-1228可抑制肿瘤细胞增殖能力、迁移和侵袭能力。此外，UTMD介导的miR-1228抑制剂递送提高了单独使用miR-1228抑制剂的转染效率。与单独敲低miR-1228相比，UTMD介导的miR-1228抑制增强了miR-1228下调对肿瘤细胞增殖能力、迁移和侵袭能力的抑制作用。
实验仅在体外的SiHa和HeLa细胞中进行，结果未在动物中验证。
这些结果表明，UTMD介导的miR-1228抑制剂递送可能是一种通过抑制细胞活性治疗宫颈癌的潜在治疗方法。
