Emerg Infect Dis. 2022 Jul;28(7):1327-1331. doi: 10.3201/eid2807.220264.
To determine molecular epidemiology and clinical features of enterovirus D68 (EV-D68) infections, we reviewed EV-D68-associated respiratory cases at a hospital in Barcelona, Spain, during 2014-2021. Respiratory samples were collected from hospitalized patients or outpatients with symptoms of acute respiratory tract infection or suggestive of enterovirus infection. Enterovirus detection was performed by real-time multiplex reverse transcription PCR and characterization by phylogenetic analysis of the partial viral protein 1 coding region sequences. From 184 patients with EV-D68 infection, circulating subclades were B3 (80%), D1 (17%), B2 (1%), and A (<1%); clade proportions shifted over time. EV-D68 was detected mostly in children (86%) and biennially (2016, 2018, 2021). In patients <16 years of age, the most common sign/symptom was lower respiratory tract infection, for which 11.8% required pediatric intensive care unit admission and 2.3% required invasive mechanical ventilation; neurologic complications developed in 1. The potential neurotropism indicates that enterovirus surveillance should be mandatory.
为了确定肠道病毒 D68（EV-D68）感染的分子流行病学和临床特征，我们回顾了西班牙巴塞罗那一家医院在 2014 年至 2021 年期间与 EV-D68 相关的呼吸道病例。从有急性呼吸道感染症状或疑似肠道病毒感染的住院患者或门诊患者中采集呼吸道样本。通过实时多重反转录 PCR 进行肠道病毒检测，并通过病毒蛋白 1 编码区序列部分的系统进化分析进行特征描述。从 184 例 EV-D68 感染患者中，循环亚群为 B3（80%）、D1（17%）、B2（1%）和 A（<1%）；亚群比例随时间推移而变化。EV-D68 主要在儿童（86%）和两年一次（2016 年、2018 年、2021 年）检测到。在<16 岁的患者中，最常见的症状是下呼吸道感染，其中 11.8%需要入住儿科重症监护病房，2.3%需要有创机械通气；1 例发生神经系统并发症。潜在的神经嗜性表明，肠道病毒监测应该是强制性的。
